Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 156311..156836 Replicon plasmid pYDC107_184
Accession NZ_CP025708
Organism Escherichia coli strain YDC107

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag YDC107_RS26455 Protein ID WP_001159868.1
Coordinates 156311..156616 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag YDC107_RS26460 Protein ID WP_000813634.1
Coordinates 156618..156836 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YDC107_RS26440 152221..153387 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
YDC107_RS26445 153975..154730 - 756 WP_000852146.1 replication initiation protein RepE -
YDC107_RS26450 155504..156310 - 807 WP_000016982.1 site-specific integrase -
YDC107_RS26455 156311..156616 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
YDC107_RS26460 156618..156836 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
YDC107_RS26465 157470..157667 + 198 WP_000215657.1 hypothetical protein -
YDC107_RS26470 157664..157948 - 285 WP_000642771.1 hypothetical protein -
YDC107_RS26475 157968..159101 - 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / aadA5 / qacE / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..184098 184098


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T91819 WP_001159868.1 NZ_CP025708:c156616-156311 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T91819 NZ_CP025708:c156616-156311 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT91819 WP_000813634.1 NZ_CP025708:c156836-156618 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT91819 NZ_CP025708:c156836-156618 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References