Detailed information of TA system TA091812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 72668..73259
• Replicon: plasmid pYDC107_184
• Accession: NZ_CP025708
• Organism: Escherichia coli strain YDC107

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q1A2D9
• Locus tag: YDC107_RS25880
• Protein ID: WP_000439434.1
• Coordinates: 72668..73000 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: V0TH33
• Locus tag: YDC107_RS25885
• Protein ID: WP_000557619.1
• Coordinates: 73002..73259 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YDC107_RS25865	68112..68816	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
YDC107_RS25870	68882..70396	+	1515	Protein_101	Tn3-like element TnAs1 family transposase	-
YDC107_RS25875	70611..72383	-	1773	WP_001262767.1	DUF262 domain-containing protein	-
YDC107_RS25880	72668..73000	-	333	WP_000439434.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
YDC107_RS25885	73002..73259	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	Antitoxin
YDC107_RS25890	73352..74005	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
YDC107_RS25895	74195..74581	-	387	WP_012311408.1	hypothetical protein	-
YDC107_RS25900	74944..75801	-	858	WP_000130948.1	incFII family plasmid replication initiator RepA	-
YDC107_RS25905	75794..75868	-	75	WP_001365705.1	RepA leader peptide Tap	-
YDC107_RS25910	75952..76068	+	117	WP_074446712.1	replication protein RepA	-
YDC107_RS25915	76104..76361	-	258	WP_000083821.1	replication regulatory protein RepA	-
YDC107_RS25920	76328..76579	+	252	WP_071529016.1	hypothetical protein	-
YDC107_RS25925	76766..77788	+	1023	WP_000156883.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / aadA5 / qacE / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14	-	1..184098	184098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11880.78 Da
• Isoelectric Point: 11.1995

>T91812 WP_000439434.1 NZ_CP025708:c73000-72668 [Escherichia coli]
MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTI
DMKARGGKRLERVPETIMNEVLGRLSTILT

Nucleotide

• Length: 333 bp

>T91812 NZ_CP025708:c73000-72668 [Escherichia coli]
ATGGAAAGAGGGGAAATCTGGCTTGTCTCGCTTGATCCTACCGCAGGTCATGAGCAGCAGGGAACGCGGCCGGTGCTGAT
TGTCACACCGGCGGCCTTTAATCGCGTGACCCGCCTGCCTGTTGTTGTGCCCGTAACCAGCGGAGGCAATTTTGCCCGCA
CTGCCGGCTTTGCGGTGTCGTTGGATGGTGTTGGCATACGTACCACAGGTGTTGTACGTTGCGATCAACCCCGGACAATT
GATATGAAAGCACGGGGCGGAAAACGACTCGAACGGGTTCCGGAGACTATCATGAACGAAGTTCTTGGCCGCCTGTCCAC
TATTCTGACTTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9332.61 Da
• Isoelectric Point: 4.2694

>AT91812 WP_000557619.1 NZ_CP025708:c73259-73002 [Escherichia coli]
MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVEPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPAT
GQEEI

Nucleotide

• Length: 258 bp

>AT91812 NZ_CP025708:c73259-73002 [Escherichia coli]
ATGCATACCACCCGACTGAAGAGGGTTGGCGGCTCAGTTATGCTGACCGTCCCACCGGCACTGCTGAATGCGCTGTCTCT
GGGCACAGATAATGAAGTTGGCATGGTCATTGATAATGGCCGGCTGATTGTTGAGCCGTACAGACGCCCGCAATATTCAC
TGGCTGAGCTACTGGCACAGTGTGATCCGAATGCTGAAATATCAGCTGAAGAACGAGAATGGCTGGATGCACCGGCGACT
GGTCAGGAGGAAATCTGA

Structures

Toxin

• AlphaFold DB: Q1A2D9

Antitoxin

• AlphaFold DB: V0TH33