Detailed information of TA system TA091775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 51514..52040
• Replicon: chromosome
• Accession: NZ_CP025707
• Organism: Escherichia coli strain YDC107

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: YDC107_RS00345
• Protein ID: WP_000323025.1
• Coordinates: 51514..51801 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: YDC107_RS00350
• Protein ID: WP_000534858.1
• Coordinates: 51801..52040 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YDC107_RS00300	46538..46753	-	216	WP_000839590.1	phage lysis protein EssD	-
YDC107_RS00305	47507..47722	-	216	WP_000066484.1	cold shock-like protein CspB	-
YDC107_RS00310	48023..48235	+	213	WP_000087756.1	cold shock-like protein CspF	-
YDC107_RS00315	48290..48379	+	90	WP_120795389.1	hypothetical protein	-
YDC107_RS00320	48657..49409	-	753	Protein_63	antitermination protein	-
YDC107_RS00325	49423..50472	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
YDC107_RS00330	50474..50752	-	279	WP_012304870.1	hypothetical protein	-
YDC107_RS00335	50819..51070	-	252	WP_000980994.1	hypothetical protein	-
YDC107_RS00340	51287..51442	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
YDC107_RS00345	51514..51801	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
YDC107_RS00350	51801..52040	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
YDC107_RS00355	52065..52370	+	306	WP_001326990.1	hypothetical protein	-
YDC107_RS00360	52573..52905	+	333	WP_001301033.1	protein FlxA	-
YDC107_RS00365	53342..54655	-	1314	WP_105955755.1	ISNCY family transposase	-
YDC107_RS00370	54833..55015	+	183	WP_000955178.1	hypothetical protein	-
YDC107_RS00375	54990..55169	-	180	WP_000786209.1	hypothetical protein	-
YDC107_RS00380	55933..56337	+	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
YDC107_RS00385	56334..56681	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	156..68827	68671

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T91775 WP_000323025.1 NZ_CP025707:c51801-51514 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T91775 NZ_CP025707:c51801-51514 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT91775 WP_000534858.1 NZ_CP025707:c52040-51801 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT91775 NZ_CP025707:c52040-51801 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8