Detailed information of TA system TA091765

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4974386..4975221
• Replicon: chromosome
• Accession: NZ_CP025703
• Organism: Escherichia coli strain BH100N substr. MG2017

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: BH100N_RS25290
• Protein ID: WP_001094426.1
• Coordinates: 4974844..4975221 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: BH100N_RS25285
• Protein ID: WP_001285575.1
• Coordinates: 4974386..4974754 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100N_RS25250	4970278..4970958	+	681	WP_001282919.1	WYL domain-containing protein	-
BH100N_RS25255	4971106..4971783	+	678	WP_001097301.1	hypothetical protein	-
BH100N_RS25260	4971789..4972022	+	234	WP_001278290.1	DUF905 family protein	-
BH100N_RS25265	4972112..4972930	+	819	WP_065377599.1	DUF945 domain-containing protein	-
BH100N_RS25270	4973022..4973507	+	486	WP_000214415.1	antirestriction protein	-
BH100N_RS25275	4973522..4973998	+	477	WP_001298074.1	RadC family protein	-
BH100N_RS25280	4974085..4974306	+	222	WP_000692350.1	DUF987 domain-containing protein	-
BH100N_RS25285	4974386..4974754	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BH100N_RS25290	4974844..4975221	+	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
BH100N_RS25295	4975218..4975706	+	489	WP_000761683.1	hypothetical protein	-
BH100N_RS25300	4975718..4975915	+	198	WP_000839272.1	DUF957 domain-containing protein	-
BH100N_RS26375	4975994..4976152	+	159	WP_001467148.1	hypothetical protein	-
BH100N_RS25310	4977100..4979136	+	2037	WP_000417001.1	hypothetical protein	-
BH100N_RS25315	4979535..4979714	-	180	WP_001298063.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4964059..4989266	25207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T91765 WP_001094426.1 NZ_CP025703:4974844-4975221 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T91765 NZ_CP025703:4974844-4975221 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT91765 WP_001285575.1 NZ_CP025703:4974386-4974754 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT91765 NZ_CP025703:4974386-4974754 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5