Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4688483..4688980 Replicon chromosome
Accession NZ_CP025703
Organism Escherichia coli strain BH100N substr. MG2017

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag BH100N_RS23810 Protein ID WP_001173343.1
Coordinates 4688807..4688980 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag BH100N_RS23805 Protein ID WP_001312331.1
Coordinates 4688483..4688779 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH100N_RS23785 4685212..4685931 - 720 WP_000611288.1 two-component system response regulator DcuR -
BH100N_RS23790 4685928..4687559 - 1632 WP_001216466.1 sensor histidine kinase -
BH100N_RS23795 4687740..4687970 + 231 WP_000373291.1 (4Fe-4S)-binding protein -
BH100N_RS23800 4687982..4688254 + 273 WP_000405647.1 N-acetyltransferase -
BH100N_RS23805 4688483..4688779 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
BH100N_RS23810 4688807..4688980 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
BH100N_RS23815 4689099..4690616 - 1518 WP_000003805.1 lysine--tRNA ligase -
BH100N_RS23820 4690853..4692310 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T91764 WP_001173343.1 NZ_CP025703:4688807-4688980 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T91764 NZ_CP025703:4688807-4688980 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT91764 WP_001312331.1 NZ_CP025703:4688483-4688779 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT91764 NZ_CP025703:4688483-4688779 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References