Detailed information of TA system TA091759

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3360015..3360849
• Replicon: chromosome
• Accession: NZ_CP025703
• Organism: Escherichia coli strain BH100N substr. MG2017

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A838AQY5
• Locus tag: BH100N_RS17145
• Protein ID: WP_023152077.1
• Coordinates: 3360472..3360849 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BH100N_RS17140
• Protein ID: WP_065377585.1
• Coordinates: 3360015..3360383 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100N_RS17105	3356830..3357285	+	456	WP_000581504.1	hypothetical protein	-
BH100N_RS17110	3357364..3357597	+	234	WP_001119729.1	DUF905 family protein	-
BH100N_RS17120	3357697..3358515	+	819	WP_032250973.1	DUF945 domain-containing protein	-
BH100N_RS17125	3358597..3359076	+	480	WP_023568406.1	antirestriction protein	-
BH100N_RS17130	3359092..3359568	+	477	WP_001186773.1	RadC family protein	-
BH100N_RS17135	3359631..3359852	+	222	WP_000692305.1	DUF987 domain-containing protein	-
BH100N_RS17140	3360015..3360383	+	369	WP_065377585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
BH100N_RS17145	3360472..3360849	+	378	WP_023152077.1	type IV toxin-antitoxin system toxin CbtA	Toxin
BH100N_RS17150	3360846..3361334	+	489	WP_065377586.1	hypothetical protein	-
BH100N_RS17155	3361346..3361543	+	198	WP_000839281.1	DUF957 domain-containing protein	-
BH100N_RS17160	3361640..3362149	+	510	WP_050582268.1	DUF4942 domain-containing protein	-
BH100N_RS17165	3362552..3362722	+	171	Protein_3135	IS110 family transposase	-
BH100N_RS17170	3363521..3364504	+	984	WP_001331698.1	KpsF/GutQ family sugar-phosphate isomerase	-
BH100N_RS17175	3364576..3365724	+	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3362618..3362722	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14056.12 Da
• Isoelectric Point: 7.8839

>T91759 WP_023152077.1 NZ_CP025703:3360472-3360849 [Escherichia coli]
MKTLPVLPGQVASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRAIGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T91759 NZ_CP025703:3360472-3360849 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGTGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAATCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13580.37 Da
• Isoelectric Point: 5.0580

>AT91759 WP_065377585.1 NZ_CP025703:3360015-3360383 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT91759 NZ_CP025703:3360015-3360383 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A838AQY5