Detailed information of TA system TA091716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 81486..82008
• Replicon: plasmid pL5201
• Accession: NZ_CP025683
• Organism: Klebsiella pneumoniae strain L5-2

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: C0067_RS00455
• Protein ID: WP_004187019.1
• Coordinates: 81724..82008 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: C0067_RS00450
• Protein ID: WP_004187025.1
• Coordinates: 81486..81734 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0067_RS00425	78853..79422	-	570	WP_004152117.1	small heat shock protein sHSP20	-
C0067_RS00430	79457..79738	-	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
C0067_RS26515	79982..80245	-	264	WP_004118208.1	hypothetical protein	-
C0067_RS26520	80260..80523	+	264	WP_004187027.1	transposase	-
C0067_RS00440	80468..80584	+	117	Protein_80	transposase domain-containing protein	-
C0067_RS00445	80772..81196	+	425	Protein_81	IS1 family transposase	-
C0067_RS00450	81486..81734	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
C0067_RS00455	81724..82008	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C0067_RS00460	82024..83379	-	1356	WP_004187017.1	ISNCY family transposase	-
C0067_RS27475	83461..83760	-	300	Protein_85	conjugal transfer protein TraH	-
C0067_RS27480	83758..84241	-	484	Protein_86	hypothetical protein	-
C0067_RS00470	84258..84782	-	525	Protein_87	hypothetical protein	-
C0067_RS00480	86325..86789	+	465	WP_004187010.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkF / mrkJ	1..139211	139211
	-	flank	IS/Tn	-	-	80966..81232	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T91716 WP_004187019.1 NZ_CP025683:81724-82008 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T91716 NZ_CP025683:81724-82008 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT91716 WP_004187025.1 NZ_CP025683:81486-81734 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT91716 NZ_CP025683:81486-81734 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4