Detailed information of TA system TA091715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 67855..68591
• Replicon: plasmid pL5201
• Accession: NZ_CP025683
• Organism: Klebsiella pneumoniae strain L5-2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: C0067_RS00360
• Protein ID: WP_004187044.1
• Coordinates: 67855..68337 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: C0067_RS00365
• Protein ID: WP_003026799.1
• Coordinates: 68325..68591 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0067_RS00340	63879..64815	-	937	Protein_60	ISNCY family transposase	-
C0067_RS00345	64925..65095	+	171	Protein_61	transposase	-
C0067_RS27470	65093..65200	+	108	Protein_62	IS3 family transposase	-
C0067_RS00350	65434..66138	-	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
C0067_RS00355	66298..67644	-	1347	WP_077251107.1	ISNCY family transposase	-
C0067_RS00360	67855..68337	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
C0067_RS00365	68325..68591	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
C0067_RS00375	68742..69446	+	705	WP_060579418.1	IS6-like element IS26 family transposase	-
C0067_RS00380	69605..72190	-	2586	WP_004187040.1	EAL domain-containing protein	-
C0067_RS00385	72159..72404	-	246	WP_032238678.1	hypothetical protein	-
C0067_RS00390	72462..73442	-	981	Protein_70	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkF / mrkJ	1..139211	139211
	-	inside	IScluster/Tn	-	-	63858..73442	9584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T91715 WP_004187044.1 NZ_CP025683:c68337-67855 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T91715 NZ_CP025683:c68337-67855 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT91715 WP_003026799.1 NZ_CP025683:c68591-68325 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT91715 NZ_CP025683:c68591-68325 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66