Detailed information of TA system TA091700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 961916..962475
• Replicon: chromosome
• Accession: NZ_CP025680
• Organism: Brucella melitensis strain CIIMS-BH-2

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: CX678_RS05015
• Protein ID: WP_002966807.1
• Coordinates: 962230..962475 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: CX678_RS05010
• Protein ID: WP_004683740.1
• Coordinates: 961916..962230 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CX678_RS04960	957620..957823	-	204	WP_004683737.1	hypothetical protein	-
CX678_RS04965	957826..958041	-	216	WP_005968947.1	hypothetical protein	-
CX678_RS04970	958038..958241	-	204	WP_002964088.1	hypothetical protein	-
CX678_RS04975	958291..958996	+	706	Protein_915	hypothetical protein	-
CX678_RS04980	958993..959223	+	231	WP_002964091.1	hypothetical protein	-
CX678_RS04985	959220..959495	+	276	WP_002964092.1	hypothetical protein	-
CX678_RS04990	959518..959994	+	477	WP_002966806.1	hypothetical protein	-
CX678_RS04995	960340..961050	+	711	WP_004683739.1	porin family protein	-
CX678_RS05000	961398..961568	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
CX678_RS05005	961567..961800	+	234	WP_002969999.1	hypothetical protein	-
CX678_RS05010	961916..962230	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
CX678_RS05015	962230..962475	-	246	WP_002966807.1	BrnT family toxin	Toxin
CX678_RS05020	962608..963246	+	639	Protein_924	tRNA-guanine transglycosylase	-
CX678_RS05025	963316..964074	+	759	Protein_925	IS5 family transposase	-
CX678_RS05030	964075..964767	+	693	WP_006135935.1	hypothetical protein	-
CX678_RS05035	965179..966630	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	955848..969946	14098
	-	flank	IS/Tn	-	-	963316..963684	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T91700 WP_002966807.1 NZ_CP025680:c962475-962230 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T91700 NZ_CP025680:c962475-962230 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT91700 WP_004683740.1 NZ_CP025680:c962230-961916 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT91700 NZ_CP025680:c962230-961916 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1