Detailed information of TA system TA091698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 22996..23489
• Replicon: plasmid pEA-3
• Accession: NZ_CP025679
• Organism: Escherichia albertii strain ChinaSP140150

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: C0R48_RS26650
• Protein ID: WP_001230709.1
• Coordinates: 23235..23489 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: C0R48_RS26645
• Protein ID: WP_000151456.1
• Coordinates: 22996..23238 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0R48_RS26600	18220..18927	+	708	WP_000108794.1	hypothetical protein	-
C0R48_RS26605	18947..19387	+	441	WP_023223352.1	hypothetical protein	-
C0R48_RS26610	19389..19715	+	327	WP_000206697.1	hypothetical protein	-
C0R48_RS26615	19975..20274	-	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
C0R48_RS26620	20776..20958	+	183	WP_001428470.1	hypothetical protein	-
C0R48_RS26625	21046..21393	-	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	-
C0R48_RS26630	21390..21638	-	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	-
C0R48_RS26635	22132..22425	-	294	WP_000673428.1	hypothetical protein	-
C0R48_RS26645	22996..23238	-	243	WP_000151456.1	hypothetical protein	Antitoxin
C0R48_RS26650	23235..23489	-	255	WP_001230709.1	hypothetical protein	Toxin
C0R48_RS26655	23486..24028	-	543	WP_001083904.1	hypothetical protein	-
C0R48_RS26660	24132..24647	-	516	WP_001025393.1	J domain-containing protein	-
C0R48_RS26665	25051..25379	-	329	Protein_29	hypothetical protein	-
C0R48_RS26675	25968..26633	+	666	WP_000532148.1	P-loop NTPase	-
C0R48_RS27410	26707..26991	+	285	WP_001162371.1	hypothetical protein	-
C0R48_RS26685	27009..27605	-	597	WP_000125165.1	hypothetical protein	-
C0R48_RS26690	27755..27916	-	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
C0R48_RS27415	28300..28461	-	162	WP_001128311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..68747	68747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T91698 WP_001230709.1 NZ_CP025679:c23489-23235 [Escherichia albertii]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T91698 NZ_CP025679:c23489-23235 [Escherichia albertii]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT91698 WP_000151456.1 NZ_CP025679:c23238-22996 [Escherichia albertii]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT91698 NZ_CP025679:c23238-22996 [Escherichia albertii]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5