Detailed information of TA system TA091697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 21046..21638
• Replicon: plasmid pEA-3
• Accession: NZ_CP025679
• Organism: Escherichia albertii strain ChinaSP140150

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: C0R48_RS26630
• Protein ID: WP_000513532.1
• Coordinates: 21390..21638 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: C0R48_RS26625
• Protein ID: WP_000681609.1
• Coordinates: 21046..21393 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0R48_RS26585	16336..16677	-	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-
C0R48_RS26590	17047..17447	+	401	Protein_15	zinc transporter	-
C0R48_RS26595	17465..18218	+	754	Protein_16	conjugal transfer protein TraL	-
C0R48_RS26600	18220..18927	+	708	WP_000108794.1	hypothetical protein	-
C0R48_RS26605	18947..19387	+	441	WP_023223352.1	hypothetical protein	-
C0R48_RS26610	19389..19715	+	327	WP_000206697.1	hypothetical protein	-
C0R48_RS26615	19975..20274	-	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
C0R48_RS26620	20776..20958	+	183	WP_001428470.1	hypothetical protein	-
C0R48_RS26625	21046..21393	-	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C0R48_RS26630	21390..21638	-	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
C0R48_RS26635	22132..22425	-	294	WP_000673428.1	hypothetical protein	-
C0R48_RS26645	22996..23238	-	243	WP_000151456.1	hypothetical protein	-
C0R48_RS26650	23235..23489	-	255	WP_001230709.1	hypothetical protein	-
C0R48_RS26655	23486..24028	-	543	WP_001083904.1	hypothetical protein	-
C0R48_RS26660	24132..24647	-	516	WP_001025393.1	J domain-containing protein	-
C0R48_RS26665	25051..25379	-	329	Protein_29	hypothetical protein	-
C0R48_RS26675	25968..26633	+	666	WP_000532148.1	P-loop NTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..68747	68747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T91697 WP_000513532.1 NZ_CP025679:c21638-21390 [Escherichia albertii]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T91697 NZ_CP025679:c21638-21390 [Escherichia albertii]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT91697 WP_000681609.1 NZ_CP025679:c21393-21046 [Escherichia albertii]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT91697 NZ_CP025679:c21393-21046 [Escherichia albertii]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49