Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 80217..80739 Replicon plasmid pEA-1
Accession NZ_CP025677
Organism Escherichia albertii strain ChinaSP140150

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag C0R48_RS25740 Protein ID WP_000220561.1
Coordinates 80458..80739 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag C0R48_RS25735 Protein ID WP_000121743.1
Coordinates 80217..80468 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0R48_RS25715 76340..76762 - 423 WP_179292036.1 DNA distortion polypeptide 3 -
C0R48_RS25720 76830..77276 - 447 WP_001074384.1 hypothetical protein -
C0R48_RS25725 77280..78122 - 843 WP_000435064.1 replication initiation protein -
C0R48_RS25730 78143..78982 - 840 WP_024129959.1 replication initiation protein -
C0R48_RS25735 80217..80468 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
C0R48_RS25740 80458..80739 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C0R48_RS27185 80978..81070 - 93 Protein_88 IS6 family transposase -
C0R48_RS25750 81236..82435 - 1200 WP_072648941.1 IS91 family transposase -
C0R48_RS25755 82445..82633 - 189 WP_000957857.1 hypothetical protein -
C0R48_RS25760 82753..84183 + 1431 Protein_91 IS4-like element IS50R family transposase -
C0R48_RS25765 84212..85006 + 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
C0R48_RS25770 85027..85263 + 237 Protein_93 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-IIa / oqxB / oqxA / blaCTX-M-55 - 1..129356 129356
- flank IS/Tn - - 74655..75854 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T91685 WP_000220561.1 NZ_CP025677:80458-80739 [Escherichia albertii]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T91685 NZ_CP025677:80458-80739 [Escherichia albertii]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT91685 WP_000121743.1 NZ_CP025677:80217-80468 [Escherichia albertii]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT91685 NZ_CP025677:80217-80468 [Escherichia albertii]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References