Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 42750..43393 Replicon plasmid pNDM5_020007
Accession NZ_CP025626
Organism Escherichia coli strain SCEC020007

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag C0634_RS00855 Protein ID WP_001034044.1
Coordinates 42977..43393 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag C0634_RS00850 Protein ID WP_001261286.1
Coordinates 42750..42980 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0634_RS00825 39381..40136 - 756 WP_000852146.1 replication initiation protein RepE -
C0634_RS00830 40858..41664 - 807 WP_000016970.1 site-specific integrase -
C0634_RS00835 41665..41970 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
C0634_RS00840 41972..42190 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
C0634_RS00850 42750..42980 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C0634_RS00855 42977..43393 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
C0634_RS00860 43468..45033 + 1566 WP_001128474.1 AAA family ATPase -
C0634_RS00865 45018..46040 + 1023 WP_000361402.1 helicase UvrD -
C0634_RS00875 46294..46991 - 698 Protein_51 IS1-like element IS1A family transposase -
C0634_RS00885 47141..47838 - 698 Protein_52 IS1 family transposase -
C0634_RS26630 48038..48151 + 114 Protein_53 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aadA5 / qacE / sul1 / blaNDM-5 / dfrA12 / aadA2 / mph(A) / blaTEM-1B / rmtB - 1..144225 144225


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T91629 WP_001034044.1 NZ_CP025626:42977-43393 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T91629 NZ_CP025626:42977-43393 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT91629 WP_001261286.1 NZ_CP025626:42750-42980 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT91629 NZ_CP025626:42750-42980 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References