Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 41665..42190 Replicon plasmid pNDM5_020007
Accession NZ_CP025626
Organism Escherichia coli strain SCEC020007

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C0634_RS00835 Protein ID WP_001159871.1
Coordinates 41665..41970 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag C0634_RS00840 Protein ID WP_000813630.1
Coordinates 41972..42190 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0634_RS00820 37627..38793 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
C0634_RS00825 39381..40136 - 756 WP_000852146.1 replication initiation protein RepE -
C0634_RS00830 40858..41664 - 807 WP_000016970.1 site-specific integrase -
C0634_RS00835 41665..41970 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C0634_RS00840 41972..42190 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C0634_RS00850 42750..42980 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
C0634_RS00855 42977..43393 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
C0634_RS00860 43468..45033 + 1566 WP_001128474.1 AAA family ATPase -
C0634_RS00865 45018..46040 + 1023 WP_000361402.1 helicase UvrD -
C0634_RS00875 46294..46991 - 698 Protein_51 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aadA5 / qacE / sul1 / blaNDM-5 / dfrA12 / aadA2 / mph(A) / blaTEM-1B / rmtB - 1..144225 144225


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T91628 WP_001159871.1 NZ_CP025626:c41970-41665 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T91628 NZ_CP025626:c41970-41665 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT91628 WP_000813630.1 NZ_CP025626:c42190-41972 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT91628 NZ_CP025626:c42190-41972 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References