Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 5022874..5023488 Replicon chromosome
Accession NZ_CP025624
Organism Pseudomonas sp. NC02

Toxin (Protein)


Gene name hicA Uniprot ID A0A246FXT5
Locus tag C0058_RS23340 Protein ID WP_003210604.1
Coordinates 5023300..5023488 (-) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A3M3XRU6
Locus tag C0058_RS23335 Protein ID WP_003210603.1
Coordinates 5022874..5023278 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0058_RS23310 5017893..5018768 + 876 WP_087694460.1 urea transporter -
C0058_RS23315 5018800..5019624 + 825 WP_008434745.1 ion transporter -
C0058_RS23320 5019732..5020736 + 1005 WP_003210600.1 sulfate ABC transporter substrate-binding protein -
C0058_RS23325 5020878..5021492 - 615 WP_102369654.1 hypothetical protein -
C0058_RS23330 5021671..5022867 + 1197 WP_032899318.1 MFS transporter -
C0058_RS23335 5022874..5023278 - 405 WP_003210603.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C0058_RS23340 5023300..5023488 - 189 WP_003210604.1 type II toxin-antitoxin system HicA family toxin Toxin
C0058_RS23345 5023705..5024583 + 879 WP_087694462.1 LysR family transcriptional regulator -
C0058_RS23350 5024587..5025696 - 1110 WP_102369655.1 cytochrome P450 -
C0058_RS23355 5025771..5026478 + 708 WP_003210607.1 metalloregulator ArsR/SmtB family transcription factor -
C0058_RS23360 5026454..5027914 - 1461 WP_102369656.1 M10 family metallopeptidase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7193.34 Da        Isoelectric Point: 11.2000

>T91622 WP_003210604.1 NZ_CP025624:c5023488-5023300 [Pseudomonas sp. NC02]
VQSRLLIKELQEAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPQGTVRSIRKRAGLFSF

Download         Length: 189 bp

>T91622 NZ_CP025624:c5023488-5023300 [Pseudomonas sp. NC02]
GTGCAAAGCAGGCTATTGATCAAAGAGCTGCAAGAGGCGGGTTGGGTGCTGGATCGGGTGACAGGCAGCCATCATATTTT
CACCCACCGCTATAACCCGTACACGATACCGGTGCCCCATCCCAAGAAGGATCTGCCCCAGGGCACCGTGCGAAGTATCA
GGAAGCGCGCCGGGCTGTTTTCGTTTTAG

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14800.88 Da        Isoelectric Point: 4.5106

>AT91622 WP_003210603.1 NZ_CP025624:c5023278-5022874 [Pseudomonas sp. NC02]
MQYPICIEWGDDFVATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHILLQEIAAEGRVIPMPTSVAAHHDNEEYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRL

Download         Length: 405 bp

>AT91622 NZ_CP025624:c5023278-5022874 [Pseudomonas sp. NC02]
ATGCAATACCCAATCTGTATCGAATGGGGTGATGATTTTGTCGCCACCGGTATTCAGATCCCCGATATTCCAGGTGCTGT
AACCGCCGGGGATAGTTTTGAAGAGGCTTACAACGCAGCGGTTGAAGTGGCGCATATCTTGTTGCAGGAGATCGCGGCAG
AGGGTCGGGTGATTCCGATGCCAACGTCGGTGGCCGCCCACCACGATAATGAGGAATACGCCGGGATGGGCTGGGGGATG
TTGGAGCTCGACATCTCGCCGTATCTGGGCAAGACCGAGAAGGTCAATGTCACCTTGCCCGGCTATGTGATCCAGCGTAT
CGACCGGTATGTGCGTGAGCACAAAGTGAAAAGCCGCTCGTCATTTCTGGCGGATGCGGCTTTGGAGAAGTTGGTGCGGT
TGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246FXT5

Antitoxin

Source ID Structure
AlphaFold DB A0A3M3XRU6

References