Detailed information of TA system TA091619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1535413..1536017
• Replicon: chromosome
• Accession: NZ_CP025624
• Organism: Pseudomonas sp. NC02

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C0058_RS07125
• Protein ID: WP_063032679.1
• Coordinates: 1535724..1536017 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A1R0GAX7
• Locus tag: C0058_RS07120
• Protein ID: WP_003212064.1
• Coordinates: 1535413..1535721 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0058_RS07090	1530923..1531255	-	333	WP_003212076.1	hypothetical protein	-
C0058_RS07095	1531278..1531688	-	411	WP_003212074.1	type II toxin-antitoxin system HicB family antitoxin	-
C0058_RS07100	1531712..1531894	-	183	WP_003189411.1	type II toxin-antitoxin system HicA family toxin	-
C0058_RS07105	1532066..1532974	-	909	WP_087692622.1	LysR family transcriptional regulator	-
C0058_RS07110	1533070..1534233	+	1164	WP_102368280.1	MFS transporter	-
C0058_RS07115	1534230..1535420	+	1191	WP_102368281.1	MFS transporter	-
C0058_RS07120	1535413..1535721	-	309	WP_003212064.1	putative addiction module antidote protein	Antitoxin
C0058_RS07125	1535724..1536017	-	294	WP_063032679.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C0058_RS07130	1536179..1536991	-	813	WP_102368282.1	helix-turn-helix transcriptional regulator	-
C0058_RS07135	1537087..1537983	+	897	WP_102368283.1	SDR family oxidoreductase	-
C0058_RS07140	1538301..1538540	+	240	WP_003212057.1	hypothetical protein	-
C0058_RS07145	1538709..1539515	+	807	WP_087692616.1	polysaccharide lyase family 7 protein	-
C0058_RS07150	1539738..1540511	+	774	WP_102368284.1	2OG-Fe dioxygenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-96)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10759.39 Da
• Isoelectric Point: 11.0685

>T91619 WP_063032679.1 NZ_CP025624:c1536017-1535724 [Pseudomonas sp. NC02]
VNYLVQQTQTFITWHSAIRDLRAKIAIARRIDRASAGNLGDIKTVGNGVSEMRVDIGLGYRVYFTQRGRTVIVLLAGGDK
SSQDADIRRAKKLAKEV

Nucleotide

• Length: 294 bp

>T91619 NZ_CP025624:c1536017-1535724 [Pseudomonas sp. NC02]
GTGAACTATCTAGTCCAGCAAACGCAGACCTTCATTACCTGGCATTCAGCAATACGTGATTTACGCGCAAAAATCGCCAT
CGCCCGGCGCATCGATCGCGCATCTGCTGGCAATCTGGGCGATATAAAGACGGTAGGCAATGGTGTCTCGGAAATGCGCG
TAGATATCGGTCTGGGTTATCGAGTGTATTTCACGCAGCGAGGAAGAACCGTCATCGTCTTGCTGGCCGGTGGCGACAAA
TCCTCACAAGACGCCGACATCCGGCGAGCAAAAAAACTGGCAAAGGAAGTTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10981.49 Da
• Isoelectric Point: 5.0484

>AT91619 WP_003212064.1 NZ_CP025624:c1535721-1535413 [Pseudomonas sp. NC02]
MSQTLTTFDMAALLDSDEAISEYLSQVLADGDNEEFLRAVGYVAKARGMARVATDTGMGRESLYKAFAPGAKPRFETVLK
VLHALGIDLQAQPGHVANHSII

Nucleotide

• Length: 309 bp

>AT91619 NZ_CP025624:c1535721-1535413 [Pseudomonas sp. NC02]
ATGAGCCAAACACTGACGACATTTGATATGGCGGCACTGCTCGACAGTGATGAAGCCATCAGCGAATACCTTTCTCAAGT
TCTGGCCGACGGCGATAACGAGGAGTTCCTCAGGGCCGTCGGGTACGTGGCCAAAGCTCGCGGGATGGCGCGGGTCGCGA
CCGATACCGGCATGGGCCGTGAGAGTCTGTACAAGGCATTTGCCCCTGGAGCAAAGCCCCGCTTCGAAACGGTACTCAAG
GTGCTTCACGCACTGGGTATCGATCTGCAAGCACAGCCGGGGCATGTCGCCAATCATTCGATTATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1R0GAX7