Detailed information of TA system TA091617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 675916..676510
• Replicon: chromosome
• Accession: NZ_CP025624
• Organism: Pseudomonas sp. NC02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C0058_RS03040
• Protein ID: WP_102368006.1
• Coordinates: 676196..676510 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N7Y2X0
• Locus tag: C0058_RS03035
• Protein ID: WP_008439033.1
• Coordinates: 675916..676203 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0058_RS03015	672292..674364	-	2073	WP_102370217.1	TonB-dependent copper receptor	-
C0058_RS03020	674458..674829	-	372	WP_003217356.1	DUF2946 domain-containing protein	-
C0058_RS03025	674894..675376	-	483	WP_102368004.1	copper chaperone PCu(A)C	-
C0058_RS03030	675423..675818	-	396	WP_102368005.1	DUF2946 domain-containing protein	-
C0058_RS03035	675916..676203	-	288	WP_008439033.1	putative addiction module antidote protein	Antitoxin
C0058_RS03040	676196..676510	-	315	WP_102368006.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C0058_RS32535	676926..677126	+	201	WP_158213535.1	hypothetical protein	-
C0058_RS03045	677155..677394	+	240	WP_003217349.1	hypothetical protein	-
C0058_RS03050	677453..677941	-	489	WP_102368007.1	hypothetical protein	-
C0058_RS03055	677938..678216	-	279	WP_003217345.1	hypothetical protein	-
C0058_RS03060	678342..679067	-	726	WP_102368008.1	cobalt-precorrin-6A reductase	-
C0058_RS03065	679064..680269	-	1206	WP_102368009.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (2-94)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11924.57 Da
• Isoelectric Point: 10.1897

>T91617 WP_102368006.1 NZ_CP025624:c676510-676196 [Pseudomonas sp. NC02]
MIHFKKSKHFLEWLDSLNNKPARARVLARLNNAQSGNFGDCETVGNGVSEMRIHYGPGYRVYFTRREEVVYLLLIGGDKS
TQKRDIQRAKQIAADFEKKGVSHD

Nucleotide

• Length: 315 bp

>T91617 NZ_CP025624:c676510-676196 [Pseudomonas sp. NC02]
ATGATTCACTTCAAGAAATCCAAGCATTTCCTGGAGTGGCTTGATTCGTTAAACAATAAACCGGCCCGTGCACGCGTACT
GGCAAGGTTGAATAACGCACAATCAGGCAACTTCGGAGACTGTGAGACAGTAGGAAATGGCGTTTCGGAAATGCGCATCC
ACTACGGTCCTGGATACCGGGTTTATTTCACTCGTAGAGAGGAGGTGGTTTACCTGTTGTTGATAGGCGGCGATAAATCA
ACGCAGAAACGCGATATCCAGCGCGCTAAGCAAATTGCAGCAGACTTTGAAAAAAAAGGAGTAAGCCATGACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10280.68 Da
• Isoelectric Point: 4.4791

>AT91617 WP_008439033.1 NZ_CP025624:c676203-675916 [Pseudomonas sp. NC02]
MTEFTDFDIAEHLKTPEDMAEYLEACFEEDAGDGLLIRSALNNIARAQGMTQVARDAGLGRESLYKALSSTGNPEFATIM
KVMKALGLKLHATAG

Nucleotide

• Length: 288 bp

>AT91617 NZ_CP025624:c676203-675916 [Pseudomonas sp. NC02]
ATGACTGAATTTACAGACTTCGACATAGCGGAACACCTCAAGACTCCTGAAGATATGGCTGAGTATCTTGAAGCGTGCTT
CGAGGAGGATGCCGGTGACGGACTGCTTATTCGTTCAGCCCTCAACAACATCGCCCGCGCCCAAGGCATGACTCAAGTAG
CCCGCGACGCAGGCCTCGGACGCGAAAGTTTATATAAAGCCCTCTCCAGCACTGGCAATCCCGAGTTCGCGACTATCATG
AAGGTCATGAAAGCGCTCGGCCTGAAGCTGCACGCCACAGCTGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N7Y2X0