Detailed information of TA system TA091603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1749981..1750558
• Replicon: chromosome
• Accession: NZ_CP025609
• Organism: Xanthomonas oryzae pv. oryzae strain MAI1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C0L89_RS08770
• Protein ID: WP_196381723.1
• Coordinates: 1750262..1750558 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A411Y3T7
• Locus tag: C0L89_RS08765
• Protein ID: WP_017154976.1
• Coordinates: 1749981..1750223 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0L89_RS08745	1746143..1746727	-	585	WP_024743447.1	NAD(P)H-dependent oxidoreductase	-
C0L89_RS08750	1746827..1747843	+	1017	WP_024743446.1	LysR family transcriptional regulator	-
C0L89_RS08760	1748395..1749885	+	1491	WP_033003336.1	site-specific integrase	-
C0L89_RS08765	1749981..1750223	+	243	WP_017154976.1	hypothetical protein	Antitoxin
C0L89_RS08770	1750262..1750558	+	297	WP_196381723.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C0L89_RS08775	1750680..1751045	+	366	Protein_1592	transposase	-
C0L89_RS08780	1751111..1752207	+	1097	Protein_1593	IS3 family transposase	-
C0L89_RS08785	1752226..1752372	+	147	WP_165961480.1	integrase core domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-95)

Antitoxin

• Antitoxin PemI-like (4-76)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10706.15 Da
• Isoelectric Point: 8.0458

>T91603 WP_196381723.1 NZ_CP025609:1750262-1750558 [Xanthomonas oryzae pv. oryzae]
MDDVGGAGHEQRGDRPHLVLSSQGYNDTTSLVVCVPVTTKPKGNPFEVSLNDLNKPSVALTNQVMTADWRSRNAFPRGFA
NKAEMLQVEAKVRALLQL

Nucleotide

• Length: 297 bp

>T91603 NZ_CP025609:1750262-1750558 [Xanthomonas oryzae pv. oryzae]
TTGGATGATGTGGGTGGGGCCGGTCATGAGCAGCGTGGGGACCGGCCACACTTGGTTCTCAGTTCGCAAGGCTACAACGA
CACAACTAGTCTAGTGGTTTGCGTTCCTGTCACAACTAAACCCAAAGGCAATCCTTTTGAAGTAAGCCTTAATGATTTGA
ACAAGCCAAGTGTTGCTTTAACAAATCAGGTCATGACGGCAGATTGGCGCAGTAGAAATGCTTTTCCCCGAGGGTTTGCT
AATAAAGCTGAAATGCTCCAAGTTGAAGCCAAGGTTCGCGCTTTGCTTCAACTTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8786.89 Da
• Isoelectric Point: 4.3753

>AT91603 WP_017154976.1 NZ_CP025609:1749981-1750223 [Xanthomonas oryzae pv. oryzae]
MAVLKMWGNSAAVRIPATYLEESKFSIDEPLVITAEEGRIVIESSKPSYNLADLVAQITPENTHGFAGHSQRVGAEDIEW

Nucleotide

• Length: 243 bp

>AT91603 NZ_CP025609:1749981-1750223 [Xanthomonas oryzae pv. oryzae]
ATGGCTGTTTTGAAGATGTGGGGGAATAGTGCTGCAGTTCGAATCCCGGCGACCTATCTCGAGGAGTCCAAGTTTTCTAT
TGACGAGCCTCTTGTAATCACTGCGGAGGAAGGTCGCATTGTGATCGAGTCGAGTAAGCCGTCTTATAACCTCGCGGACT
TGGTTGCTCAAATCACTCCAGAAAACACGCACGGCTTTGCTGGGCATTCCCAGAGAGTCGGGGCGGAGGATATTGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A411Y3T7