Detailed information of TA system TA091595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3136622..3137226
• Replicon: chromosome
• Accession: NZ_CP025608
• Organism: Mycobacterium tuberculosis strain GG-229-10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: C0089_RS15475
• Protein ID: WP_003414492.1
• Coordinates: 3136622..3137014 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: C0089_RS15480
• Protein ID: WP_003414495.1
• Coordinates: 3137011..3137226 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0089_RS15445	3131773..3132560	-	788	Protein_2918	CRISPR-associated endoribonuclease Cas6	-
C0089_RS15450	3132894..3133439	-	546	WP_003913758.1	DUF1802 family protein	-
C0089_RS15455	3133711..3134595	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
C0089_RS15460	3134598..3135485	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
C0089_RS15465	3135790..3136335	-	546	WP_003899500.1	DUF1802 family protein	-
C0089_RS15470	3136332..3136601	-	270	WP_003414489.1	DUF2277 family protein	-
C0089_RS15475	3136622..3137014	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
C0089_RS15480	3137011..3137226	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C0089_RS15485	3137273..3138022	+	750	WP_070898763.1	enoyl-CoA hydratase	-
C0089_RS15490	3138101..3139183	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
C0089_RS15495	3139176..3140486	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
C0089_RS15500	3140489..3141316	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
C0089_RS15505	3141313..3142224	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T91595 WP_003414492.1 NZ_CP025608:c3137014-3136622 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T91595 NZ_CP025608:c3137014-3136622 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT91595 WP_003414495.1 NZ_CP025608:c3137226-3137011 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT91595 NZ_CP025608:c3137226-3137011 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8