Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3110148..3110718 Replicon chromosome
Accession NZ_CP025608
Organism Mycobacterium tuberculosis strain GG-229-10

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag C0089_RS15330 Protein ID WP_003414166.1
Coordinates 3110148..3110504 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag C0089_RS15335 Protein ID WP_003901465.1
Coordinates 3110488..3110718 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0089_RS15310 3105600..3107288 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
C0089_RS15315 3107292..3107618 - 327 WP_003414157.1 hypothetical protein -
C0089_RS15320 3107791..3108378 + 588 WP_023641719.1 DUF3558 family protein -
C0089_RS15325 3108397..3110046 + 1650 WP_070898762.1 CocE/NonD family hydrolase -
C0089_RS15330 3110148..3110504 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
C0089_RS15335 3110488..3110718 - 231 WP_003901465.1 antitoxin MazE Antitoxin
C0089_RS15340 3110761..3111804 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
C0089_RS15345 3111995..3112270 + 276 WP_003911993.1 DUF1778 domain-containing protein -
C0089_RS21545 3112446..3112700 - 255 WP_003917684.1 hypothetical protein -
C0089_RS15355 3112848..3113252 + 405 WP_009938577.1 hypothetical protein -
C0089_RS15360 3113249..3113440 + 192 WP_003414184.1 hypothetical protein -
C0089_RS15365 3113504..3114793 + 1290 Protein_2902 transposase family protein -
C0089_RS15370 3115027..3115284 + 258 WP_003899489.1 hypothetical protein -
C0089_RS15375 3115389..3115700 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T91593 WP_003414166.1 NZ_CP025608:c3110504-3110148 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T91593 NZ_CP025608:c3110504-3110148 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT91593 WP_003901465.1 NZ_CP025608:c3110718-3110488 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT91593 NZ_CP025608:c3110718-3110488 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References