Detailed information of TA system TA091473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3136602..3137206
• Replicon: chromosome
• Accession: NZ_CP025606
• Organism: Mycobacterium tuberculosis strain GG-137-10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: C0097_RS15480
• Protein ID: WP_003414492.1
• Coordinates: 3136602..3136994 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: C0097_RS15485
• Protein ID: WP_003414495.1
• Coordinates: 3136991..3137206 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0097_RS15450	3131752..3132540	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
C0097_RS15455	3132874..3133419	-	546	WP_003913758.1	DUF1802 family protein	-
C0097_RS15460	3133691..3134575	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
C0097_RS15465	3134578..3135465	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
C0097_RS15470	3135770..3136315	-	546	WP_003899500.1	DUF1802 family protein	-
C0097_RS15475	3136312..3136581	-	270	WP_003414489.1	DUF2277 family protein	-
C0097_RS15480	3136602..3136994	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
C0097_RS15485	3136991..3137206	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C0097_RS15490	3137253..3138002	+	750	WP_003414497.1	enoyl-CoA hydratase	-
C0097_RS15495	3138081..3139163	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
C0097_RS15500	3139156..3140466	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
C0097_RS15505	3140469..3141296	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
C0097_RS15510	3141293..3142204	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T91473 WP_003414492.1 NZ_CP025606:c3136994-3136602 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T91473 NZ_CP025606:c3136994-3136602 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT91473 WP_003414495.1 NZ_CP025606:c3137206-3136991 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT91473 NZ_CP025606:c3137206-3136991 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8