Detailed information of TA system TA091465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2868577..2869208
• Replicon: chromosome
• Accession: NZ_CP025606
• Organism: Mycobacterium tuberculosis strain GG-137-10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: C0097_RS13970
• Protein ID: WP_003413174.1
• Coordinates: 2868831..2869208 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: C0097_RS13965
• Protein ID: WP_003413167.1
• Coordinates: 2868577..2868834 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0097_RS13930	2864491..2864805	+	315	WP_009937839.1	hypothetical protein	-
C0097_RS13935	2865101..2866312	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
C0097_RS13945	2866439..2867098	+	660	WP_102770764.1	LppA family lipoprotein	-
C0097_RS13950	2867095..2867757	+	663	WP_003900848.1	hypothetical protein	-
C0097_RS13955	2867754..2868032	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
C0097_RS13960	2868125..2868538	+	414	WP_003413164.1	PIN domain nuclease	-
C0097_RS13965	2868577..2868834	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
C0097_RS13970	2868831..2869208	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
C0097_RS13975	2869224..2869598	-	375	WP_003413177.1	hypothetical protein	-
C0097_RS13980	2869698..2870093	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
C0097_RS13985	2870090..2870335	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
C0097_RS13995	2870746..2871165	-	420	WP_003413190.1	A24 family peptidase	-
C0097_RS14000	2871177..2871986	-	810	WP_003413193.1	shikimate dehydrogenase	-
C0097_RS14005	2871983..2873236	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
C0097_RS14010	2873229..2873741	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T91465 WP_003413174.1 NZ_CP025606:2868831-2869208 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T91465 NZ_CP025606:2868831-2869208 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT91465 WP_003413167.1 NZ_CP025606:2868577-2868834 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT91465 NZ_CP025606:2868577-2868834 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5