Detailed information of TA system TA091425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 640211..640887
• Replicon: chromosome
• Accession: NZ_CP025606
• Organism: Mycobacterium tuberculosis strain GG-137-10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: C0097_RS02940
• Protein ID: WP_003898500.1
• Coordinates: 640211..640624 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: C0097_RS02945
• Protein ID: WP_003402915.1
• Coordinates: 640621..640887 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0097_RS02910	635556..635858	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
C0097_RS02915	635918..636196	-	279	WP_003402901.1	hypothetical protein	-
C0097_RS02920	636193..637446	-	1254	WP_003402904.1	inorganic phosphate transporter family protein	-
C0097_RS02925	637566..637952	-	387	WP_003402907.1	VOC family protein	-
C0097_RS02930	638015..638899	-	885	WP_003402909.1	SDR family oxidoreductase	-
C0097_RS02935	638995..639897	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
C0097_RS02940	640211..640624	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
C0097_RS02945	640621..640887	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
C0097_RS02950	641079..642794	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
C0097_RS02955	642872..644476	+	1605	WP_003402920.1	amidohydrolase	-
C0097_RS02960	644473..645453	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T91425 WP_003898500.1 NZ_CP025606:c640624-640211 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T91425 NZ_CP025606:c640624-640211 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT91425 WP_003402915.1 NZ_CP025606:c640887-640621 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT91425 NZ_CP025606:c640887-640621 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0