Detailed information of TA system TA091422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 363484..364055
• Replicon: chromosome
• Accession: NZ_CP025606
• Organism: Mycobacterium tuberculosis strain GG-137-10

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: C0097_RS01625
• Protein ID: WP_003401560.1
• Coordinates: 363484..363786 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: C0097_RS01630
• Protein ID: WP_003401563.1
• Coordinates: 363834..364055 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0097_RS01605	358953..359756	-	804	WP_003401540.1	hypothetical protein	-
C0097_RS01610	359766..361163	-	1398	WP_003401544.1	sulfatase	-
C0097_RS01615	361342..363117	+	1776	WP_010886074.1	PE family protein	-
C0097_RS01620	363260..363487	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
C0097_RS01625	363484..363786	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
C0097_RS01630	363834..364055	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
C0097_RS01635	364052..364477	+	426	WP_003401566.1	PIN domain nuclease	-
C0097_RS01640	364613..365245	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
C0097_RS01645	365242..366150	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T91422 WP_003401560.1 NZ_CP025606:363484-363786 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T91422 NZ_CP025606:363484-363786 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT91422 WP_003401563.1 NZ_CP025606:363834-364055 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT91422 NZ_CP025606:363834-364055 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48