Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3110062..3110632 Replicon chromosome
Accession NZ_CP025604
Organism Mycobacterium tuberculosis strain GG-129-11

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag C0091_RS15340 Protein ID WP_003414166.1
Coordinates 3110062..3110418 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag C0091_RS15345 Protein ID WP_003901465.1
Coordinates 3110402..3110632 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0091_RS15320 3105514..3107202 - 1689 WP_010924579.1 alpha/beta hydrolase family protein -
C0091_RS15325 3107206..3107532 - 327 WP_003414157.1 hypothetical protein -
C0091_RS15330 3107705..3108292 + 588 WP_003914429.1 DUF3558 family protein -
C0091_RS15335 3108311..3109960 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
C0091_RS15340 3110062..3110418 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
C0091_RS15345 3110402..3110632 - 231 WP_003901465.1 antitoxin MazE Antitoxin
C0091_RS15350 3110675..3111718 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
C0091_RS15355 3111909..3112184 + 276 WP_003911993.1 DUF1778 domain-containing protein -
C0091_RS21565 3112360..3112614 - 255 WP_003917684.1 hypothetical protein -
C0091_RS15365 3112762..3113166 + 405 WP_009938577.1 hypothetical protein -
C0091_RS15370 3113163..3113354 + 192 WP_003414184.1 hypothetical protein -
C0091_RS15375 3113418..3114707 + 1290 Protein_2899 transposase family protein -
C0091_RS15380 3114941..3115198 + 258 WP_003899489.1 hypothetical protein -
C0091_RS15385 3115303..3115614 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T91349 WP_003414166.1 NZ_CP025604:c3110418-3110062 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T91349 NZ_CP025604:c3110418-3110062 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT91349 WP_003901465.1 NZ_CP025604:c3110632-3110402 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT91349 NZ_CP025604:c3110632-3110402 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References