Detailed information of TA system TA091282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2868582..2869213
• Replicon: chromosome
• Accession: NZ_CP025603
• Organism: Mycobacterium tuberculosis strain GG-121-10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: C0087_RS13955
• Protein ID: WP_003413174.1
• Coordinates: 2868836..2869213 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: C0087_RS13950
• Protein ID: WP_003413167.1
• Coordinates: 2868582..2868839 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0087_RS13915	2864496..2864810	+	315	WP_009937839.1	hypothetical protein	-
C0087_RS13920	2865106..2866317	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
C0087_RS13930	2866444..2867103	+	660	WP_003900846.1	LppA family lipoprotein	-
C0087_RS13935	2867100..2867762	+	663	WP_003900848.1	hypothetical protein	-
C0087_RS13940	2867759..2868037	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
C0087_RS13945	2868130..2868543	+	414	WP_003413164.1	PIN domain nuclease	-
C0087_RS13950	2868582..2868839	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
C0087_RS13955	2868836..2869213	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
C0087_RS13960	2869229..2869603	-	375	WP_003413177.1	hypothetical protein	-
C0087_RS13965	2869703..2870098	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
C0087_RS13970	2870095..2870340	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
C0087_RS13980	2870751..2871170	-	420	WP_003413190.1	A24 family peptidase	-
C0087_RS13985	2871182..2871991	-	810	WP_003413193.1	shikimate dehydrogenase	-
C0087_RS13990	2871988..2873241	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
C0087_RS13995	2873234..2873746	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T91282 WP_003413174.1 NZ_CP025603:2868836-2869213 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T91282 NZ_CP025603:2868836-2869213 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT91282 WP_003413167.1 NZ_CP025603:2868582-2868839 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT91282 NZ_CP025603:2868582-2868839 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5