Detailed information of TA system TA091160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2868631..2869262
• Replicon: chromosome
• Accession: NZ_CP025601
• Organism: Mycobacterium tuberculosis strain GG-90-10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: C0095_RS13985
• Protein ID: WP_003413174.1
• Coordinates: 2868885..2869262 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: C0095_RS13980
• Protein ID: WP_003413167.1
• Coordinates: 2868631..2868888 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0095_RS13945	2864545..2864859	+	315	WP_009937839.1	hypothetical protein	-
C0095_RS13950	2865155..2866366	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
C0095_RS13960	2866493..2867152	+	660	WP_003900846.1	LppA family lipoprotein	-
C0095_RS13965	2867149..2867811	+	663	WP_003900848.1	hypothetical protein	-
C0095_RS13970	2867808..2868086	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
C0095_RS13975	2868179..2868592	+	414	WP_003413164.1	PIN domain nuclease	-
C0095_RS13980	2868631..2868888	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
C0095_RS13985	2868885..2869262	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
C0095_RS13990	2869278..2869652	-	375	WP_003413177.1	hypothetical protein	-
C0095_RS13995	2869752..2870147	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
C0095_RS14000	2870144..2870389	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
C0095_RS14010	2870800..2871219	-	420	WP_003413190.1	A24 family peptidase	-
C0095_RS14015	2871231..2872040	-	810	WP_003413193.1	shikimate dehydrogenase	-
C0095_RS14020	2872037..2873290	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
C0095_RS14025	2873283..2873795	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T91160 WP_003413174.1 NZ_CP025601:2868885-2869262 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T91160 NZ_CP025601:2868885-2869262 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT91160 WP_003413167.1 NZ_CP025601:2868631-2868888 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT91160 NZ_CP025601:2868631-2868888 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5