Detailed information of TA system TA091117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 363461..364032
• Replicon: chromosome
• Accession: NZ_CP025601
• Organism: Mycobacterium tuberculosis strain GG-90-10

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: C0095_RS01620
• Protein ID: WP_003401560.1
• Coordinates: 363461..363763 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: C0095_RS01625
• Protein ID: WP_003401563.1
• Coordinates: 363811..364032 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0095_RS01600	358930..359733	-	804	WP_003401540.1	hypothetical protein	-
C0095_RS01605	359743..361140	-	1398	WP_003401544.1	sulfatase	-
C0095_RS01610	361319..363094	+	1776	WP_010886074.1	PE family protein	-
C0095_RS01615	363237..363464	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
C0095_RS01620	363461..363763	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
C0095_RS01625	363811..364032	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
C0095_RS01630	364029..364454	+	426	WP_003401566.1	PIN domain nuclease	-
C0095_RS01635	364590..365222	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
C0095_RS01640	365219..366127	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T91117 WP_003401560.1 NZ_CP025601:363461-363763 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T91117 NZ_CP025601:363461-363763 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT91117 WP_003401563.1 NZ_CP025601:363811-364032 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT91117 NZ_CP025601:363811-364032 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48