Detailed information of TA system TA091100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2869708..2870345
• Replicon: chromosome
• Accession: NZ_CP025600
• Organism: Mycobacterium tuberculosis strain GG-77-11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: C0088_RS13990
• Protein ID: WP_003413180.1
• Coordinates: 2869708..2870103 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: C0088_RS13995
• Protein ID: WP_003413183.1
• Coordinates: 2870100..2870345 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0088_RS13945	2865111..2866322	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
C0088_RS13955	2866449..2867108	+	660	WP_003900846.1	LppA family lipoprotein	-
C0088_RS13960	2867105..2867767	+	663	WP_003900848.1	hypothetical protein	-
C0088_RS13965	2867764..2868042	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
C0088_RS13970	2868135..2868548	+	414	WP_003413164.1	PIN domain nuclease	-
C0088_RS13975	2868587..2868844	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
C0088_RS13980	2868841..2869218	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
C0088_RS13985	2869234..2869608	-	375	WP_003413177.1	hypothetical protein	-
C0088_RS13990	2869708..2870103	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
C0088_RS13995	2870100..2870345	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
C0088_RS14005	2870756..2871175	-	420	WP_003413190.1	A24 family peptidase	-
C0088_RS14010	2871187..2871996	-	810	WP_003413193.1	shikimate dehydrogenase	-
C0088_RS14015	2871993..2873246	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
C0088_RS14020	2873239..2873751	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T91100 WP_003413180.1 NZ_CP025600:c2870103-2869708 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T91100 NZ_CP025600:c2870103-2869708 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT91100 WP_003413183.1 NZ_CP025600:c2870345-2870100 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT91100 NZ_CP025600:c2870345-2870100 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31