Detailed information of TA system TA091082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1764712..1765325
• Replicon: chromosome
• Accession: NZ_CP025600
• Organism: Mycobacterium tuberculosis strain GG-77-11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: C0088_RS08575
• Protein ID: WP_003407786.1
• Coordinates: 1764921..1765325 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: C0088_RS08570
• Protein ID: WP_009935474.1
• Coordinates: 1764712..1764915 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0088_RS08540	1760117..1760497	+	381	WP_003407768.1	fumarate reductase subunit C	-
C0088_RS08545	1760494..1760871	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
C0088_RS08550	1760939..1761547	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
C0088_RS08555	1761731..1762879	+	1149	Protein_1615	MMPL family transporter	-
C0088_RS08560	1762889..1763335	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
C0088_RS08565	1763370..1764659	+	1290	WP_003407781.1	threonine ammonia-lyase	-
C0088_RS08570	1764712..1764915	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
C0088_RS08575	1764921..1765325	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
C0088_RS08580	1765342..1767084	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
C0088_RS08585	1767077..1769374	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T91082 WP_003407786.1 NZ_CP025600:1764921-1765325 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T91082 NZ_CP025600:1764921-1765325 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT91082 WP_009935474.1 NZ_CP025600:1764712-1764915 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT91082 NZ_CP025600:1764712-1764915 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46