Detailed information of TA system TA091076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239365..1239933
• Replicon: chromosome
• Accession: NZ_CP025600
• Organism: Mycobacterium tuberculosis strain GG-77-11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: C0088_RS06125
• Protein ID: WP_003405865.1
• Coordinates: 1239559..1239933 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: C0088_RS06120
• Protein ID: WP_003405863.1
• Coordinates: 1239365..1239562 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0088_RS06100	1235406..1236044	-	639	WP_003898730.1	lipid droplet-associated protein	-
C0088_RS06105	1236134..1237141	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
C0088_RS06110	1237158..1238141	-	984	WP_003898731.1	hypothetical protein	-
C0088_RS06115	1238204..1239277	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
C0088_RS06120	1239365..1239562	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
C0088_RS06125	1239559..1239933	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
C0088_RS06130	1240136..1240834	+	699	WP_003898733.1	hypothetical protein	-
C0088_RS06135	1240952..1241137	+	186	WP_003901093.1	hypothetical protein	-
C0088_RS06140	1241064..1241339	-	276	WP_003405867.1	hypothetical protein	-
C0088_RS06145	1241582..1241905	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
C0088_RS06150	1241920..1242630	-	711	WP_003911399.1	hypothetical protein	-
C0088_RS21670	1242652..1242861	+	210	WP_003911400.1	hypothetical protein	-
C0088_RS06155	1242813..1243520	-	708	Protein_1166	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T91076 WP_003405865.1 NZ_CP025600:1239559-1239933 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T91076 NZ_CP025600:1239559-1239933 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT91076 WP_003405863.1 NZ_CP025600:1239365-1239562 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT91076 NZ_CP025600:1239365-1239562 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07