91

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/RHH-PIN
Location 1239416..1239984 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name vapC Uniprot ID P9WF73
Locus tag Rv1114 Protein ID NP_215630.1
Coordinates 1239610..1239984 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ33
Locus tag Rv1113 Protein ID NP_215629.1
Coordinates 1239416..1239613 (+) Length 66 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv1109c 1235457..1236095 - 639 NP_215625.1 hypothetical protein -
Rv1110 1236185..1237192 + 1008 YP_177788.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
Rv1111c 1237209..1238192 - 984 NP_215627.2 hypothetical protein -
Rv1112 1238255..1239328 + 1074 NP_215628.1 GTP-binding protein -
Rv1113 1239416..1239613 + 198 NP_215629.1 antitoxin VapB32 Antitoxin
Rv1114 1239610..1239984 + 375 NP_215630.1 ribonuclease VapC32 Toxin
Rv1115 1240187..1240885 + 699 NP_215631.1 hypothetical protein -
Rv1116 1241003..1241188 + 186 NP_215632.1 hypothetical protein -
Rv1117 1241633..1241956 + 324 NP_215633.1 hypothetical protein -
Rv1118c 1241971..1242831 - 861 NP_215634.1 hypothetical protein -
Rv1119c 1242864..1243013 - 150 NP_215635.1 hypothetical protein -
Rv1120c 1243010..1243504 - 495 NP_215636.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-111)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13444.56 Da        Isoelectric Point: 5.1881

>T91 NP_215630.1 NC_000962:1239610-1239984 [Mycobacterium tuberculosis H37Rv]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Download         Length: 375 bp

>T91 NC_000962:1239610-1239984 [Mycobacterium tuberculosis H37Rv]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin


Download         Length: 66 a.a.        Molecular weight: 6973.03 Da        Isoelectric Point: 11.7327

>AT91 NP_215629.1 NC_000962:1239416-1239613 [Mycobacterium tuberculosis H37Rv]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Download         Length: 198 bp

>AT91 NC_000962:1239416-1239613 [Mycobacterium tuberculosis H37Rv]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P9WF73

Antitoxin

Source ID Structure
AlphaFold DB P9WJ33

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113] experimental


experimental experimental literature