Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3070175..3070854 Replicon chromosome
Accession NZ_CP025598
Organism Mycobacterium tuberculosis strain GG-37-11

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag C0084_RS15120 Protein ID WP_003414059.1
Coordinates 3070175..3070591 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag C0084_RS15125 Protein ID WP_003414061.1
Coordinates 3070588..3070854 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0084_RS15100 3066227..3067129 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
C0084_RS15105 3067198..3067950 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
C0084_RS15110 3068194..3068469 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
C0084_RS15115 3068466..3070088 - 1623 WP_003906897.1 type I restriction-modification system subunit M -
C0084_RS15120 3070175..3070591 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
C0084_RS15125 3070588..3070854 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
C0084_RS15130 3070880..3071275 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
C0084_RS15135 3071272..3071541 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
C0084_RS15140 3071551..3072645 - 1095 WP_003414068.1 restriction endonuclease subunit S -
C0084_RS15145 3072642..3073061 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
C0084_RS15150 3073135..3073614 - 480 WP_003414073.1 dihydrofolate reductase -
C0084_RS15155 3073685..3074485 - 801 WP_009939104.1 thymidylate synthase -
C0084_RS15160 3074641..3075378 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T90981 WP_003414059.1 NZ_CP025598:c3070591-3070175 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T90981 NZ_CP025598:c3070591-3070175 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT90981 WP_003414061.1 NZ_CP025598:c3070854-3070588 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT90981 NZ_CP025598:c3070854-3070588 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References