Detailed information of TA system TA090963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194659..2195362
• Replicon: chromosome
• Accession: NZ_CP025598
• Organism: Mycobacterium tuberculosis strain GG-37-11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: C0084_RS10590
• Protein ID: WP_003409778.1
• Coordinates: 2194659..2194988 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: C0084_RS10595
• Protein ID: WP_003409780.1
• Coordinates: 2194985..2195362 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0084_RS10570	2191042..2192112	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
C0084_RS10575	2192109..2192624	+	516	WP_012054301.1	flavin reductase family protein	-
C0084_RS10580	2192621..2193682	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
C0084_RS10585	2193679..2194449	+	771	WP_003409775.1	SDR family oxidoreductase	-
C0084_RS10590	2194659..2194988	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C0084_RS10595	2194985..2195362	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
C0084_RS10600	2195359..2195949	-	591	WP_003910477.1	SEC-C domain-containing protein	-
C0084_RS10605	2196004..2197368	+	1365	WP_012054302.1	HNH endonuclease	-
C0084_RS10610	2197523..2197975	-	453	WP_003899095.1	lipoprotein	-
C0084_RS10615	2198039..2198440	+	402	WP_003409869.1	hypothetical protein	-
C0084_RS10620	2198433..2198615	-	183	WP_003409870.1	hypothetical protein	-
C0084_RS10625	2198729..2199079	-	351	WP_003899096.1	hypothetical protein	-
C0084_RS10630	2199090..2199992	-	903	WP_003899097.1	hypothetical protein	-
C0084_RS10635	2200013..2200204	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T90963 WP_003409778.1 NZ_CP025598:c2194988-2194659 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T90963 NZ_CP025598:c2194988-2194659 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT90963 WP_003409780.1 NZ_CP025598:c2195362-2194985 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT90963 NZ_CP025598:c2195362-2194985 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8