Detailed information of TA system TA090924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3136590..3137194
• Replicon: chromosome
• Accession: NZ_CP025597
• Organism: Mycobacterium tuberculosis strain GG-36-11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: C0086_RS15480
• Protein ID: WP_003414492.1
• Coordinates: 3136590..3136982 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: C0086_RS15485
• Protein ID: WP_003414495.1
• Coordinates: 3136979..3137194 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0086_RS15450	3131740..3132528	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
C0086_RS15455	3132862..3133407	-	546	WP_003913758.1	DUF1802 family protein	-
C0086_RS15460	3133679..3134563	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
C0086_RS15465	3134566..3135453	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
C0086_RS15470	3135758..3136303	-	546	WP_003899500.1	DUF1802 family protein	-
C0086_RS15475	3136300..3136569	-	270	WP_003414489.1	DUF2277 family protein	-
C0086_RS15480	3136590..3136982	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
C0086_RS15485	3136979..3137194	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C0086_RS15490	3137241..3137990	+	750	WP_003414497.1	enoyl-CoA hydratase	-
C0086_RS15495	3138069..3139151	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
C0086_RS15500	3139144..3140454	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
C0086_RS15505	3140457..3141284	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
C0086_RS15510	3141281..3142192	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T90924 WP_003414492.1 NZ_CP025597:c3136982-3136590 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T90924 NZ_CP025597:c3136982-3136590 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT90924 WP_003414495.1 NZ_CP025597:c3137194-3136979 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT90924 NZ_CP025597:c3137194-3136979 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8