Detailed information of TA system TA090819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697052..697698
• Replicon: chromosome
• Accession: NZ_CP025596
• Organism: Mycobacterium tuberculosis strain GG-27-11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C0082_RS03200
• Protein ID: WP_031702485.1
• Coordinates: 697052..697465 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: C0082_RS03205
• Protein ID: WP_003403139.1
• Coordinates: 697462..697698 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0082_RS03180	693135..694685	+	1551	WP_003403119.1	MCE family protein	-
C0082_RS03185	694737..695129	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
C0082_RS03190	695126..695383	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
C0082_RS03195	695566..696801	-	1236	WP_031702484.1	ATP-binding protein	-
C0082_RS03200	697052..697465	-	414	WP_031702485.1	type II toxin-antitoxin system VapC family toxin	Toxin
C0082_RS03205	697462..697698	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C0082_RS03210	697802..698308	-	507	WP_003900973.1	two component system sensor kinase HK1	-
C0082_RS03215	698422..698952	-	531	WP_003917320.1	two component system sensor kinase HK2	-
C0082_RS03220	698936..699631	-	696	WP_003918099.1	two-component system response regulator TcrA	-
C0082_RS03225	699754..700065	+	312	WP_003403164.1	hypothetical protein	-
C0082_RS03230	700137..701087	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
C0082_RS03235	701328..701912	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
C0082_RS03240	701914..702624	+	711	Protein_626	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14814.20 Da
• Isoelectric Point: 8.1405

>T90819 WP_031702485.1 NZ_CP025596:c697465-697052 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWVHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T90819 NZ_CP025596:c697465-697052 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGTACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT90819 WP_003403139.1 NZ_CP025596:c697698-697462 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT90819 NZ_CP025596:c697698-697462 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Antitoxin

• AlphaFold DB: G0TQ89