Detailed information of TA system TA090606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1766686..1767276
• Replicon: chromosome
• Accession: NZ_CP025576
• Organism: Klebsiella pneumoniae strain 08EU827

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: CY656_RS08745
• Protein ID: WP_023341911.1
• Coordinates: 1766944..1767276 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: CY656_RS08740
• Protein ID: WP_000288812.1
• Coordinates: 1766686..1766943 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY656_RS08715	1763021..1764769	+	1749	WP_032418698.1	hypothetical protein	-
CY656_RS08720	1764823..1765404	+	582	WP_099184326.1	hypothetical protein	-
CY656_RS08725	1765687..1766148	+	462	WP_004213450.1	hypothetical protein	-
CY656_RS08730	1766145..1766351	+	207	WP_020805021.1	helix-turn-helix domain-containing protein	-
CY656_RS08740	1766686..1766943	+	258	WP_000288812.1	antitoxin	Antitoxin
CY656_RS08745	1766944..1767276	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CY656_RS08755	1767598..1769034	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
CY656_RS08765	1769400..1770854	-	1455	WP_004148975.1	AMP nucleosidase	-
CY656_RS08770	1770984..1771229	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T90606 WP_023341911.1 NZ_CP025576:1766944-1767276 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T90606 NZ_CP025576:1766944-1767276 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT90606 WP_000288812.1 NZ_CP025576:1766686-1766943 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT90606 NZ_CP025576:1766686-1766943 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0