Detailed information of TA system TA090603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 124757..125304
• Replicon: plasmid pE1246_1
• Accession: NZ_CP025574
• Organism: Escherichia coli strain E-1246

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CY655_RS27710
• Protein ID: WP_001402901.1
• Coordinates: 125023..125304 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: CY655_RS27705
• Protein ID: WP_000079941.1
• Coordinates: 124757..125026 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY655_RS27650	120244..120804	+	561	WP_000139341.1	fertility inhibition protein FinO	-
CY655_RS27655	120936..121148	+	213	WP_001301409.1	ANR family transcriptional regulator	-
CY655_RS28010	121660..121946	+	287	Protein_144	DUF2726 domain-containing protein	-
-	121913..121964	-	52	NuclAT_1	-	-
-	121913..121964	-	52	NuclAT_1	-	-
-	121913..121964	-	52	NuclAT_1	-	-
-	121913..121964	-	52	NuclAT_1	-	-
CY655_RS27670	122017..122151	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	-
CY655_RS27675	122424..122678	+	255	WP_000083850.1	replication regulatory protein RepA	-
CY655_RS28015	122784..122918	+	135	Protein_147	protein CopA/IncA	-
CY655_RS27685	122915..122989	+	75	WP_031943482.1	RepA leader peptide Tap	-
CY655_RS27690	122982..123839	+	858	WP_032284166.1	incFII family plasmid replication initiator RepA	-
CY655_RS27705	124757..125026	+	270	WP_000079941.1	hypothetical protein	Antitoxin
CY655_RS27710	125023..125304	+	282	WP_001402901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CY655_RS27715	125350..126189	+	840	WP_032269705.1	3'-5' exonuclease	-
CY655_RS27720	126306..126788	+	483	WP_001402899.1	hypothetical protein	-
CY655_RS27725	126948..127970	+	1023	WP_096790897.1	IS110 family transposase	-
CY655_RS27730	128263..128502	+	240	Protein_155	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	agg3D / agg3C / agg3B / aap/aspU / pic	1..129195	129195
	-	flank	IS/Tn	-	-	126948..127970	1022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10875.53 Da
• Isoelectric Point: 8.4615

>T90603 WP_001402901.1 NZ_CP025574:125023-125304 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSQWGKV
YILRVLHTTQKWP

Nucleotide

• Length: 282 bp

>T90603 NZ_CP025574:125023-125304 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTGCCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTACACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT90603 WP_000079941.1 NZ_CP025574:124757-125026 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT90603 NZ_CP025574:124757-125026 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5