Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 34906..35431 Replicon plasmid pE1246_1
Accession NZ_CP025574
Organism Escherichia coli strain E-1246

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CY655_RS27085 Protein ID WP_001159871.1
Coordinates 35126..35431 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CY655_RS27080 Protein ID WP_000813634.1
Coordinates 34906..35124 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CY655_RS27060 31349..33487 + 2139 WP_032269946.1 ATPase AAA -
CY655_RS27065 33657..34058 - 402 WP_001441071.1 type II toxin-antitoxin system VapC family toxin -
CY655_RS27070 34082..34312 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CY655_RS27080 34906..35124 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CY655_RS27085 35126..35431 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CY655_RS27090 35432..36241 + 810 WP_000016979.1 site-specific integrase -
CY655_RS27095 36379..36654 - 276 WP_000239529.1 hypothetical protein -
CY655_RS27100 36648..37292 - 645 WP_000633911.1 AAA family ATPase -
CY655_RS27105 37521..38492 + 972 WP_001103689.1 hypothetical protein -
CY655_RS27110 38497..38889 + 393 WP_000340832.1 plasmid partitioning/stability family protein -
CY655_RS27115 38921..40049 - 1129 Protein_48 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - agg3D / agg3C / agg3B / aap/aspU / pic 1..129195 129195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T90594 WP_001159871.1 NZ_CP025574:35126-35431 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T90594 NZ_CP025574:35126-35431 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT90594 WP_000813634.1 NZ_CP025574:34906-35124 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT90594 NZ_CP025574:34906-35124 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References