90594

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 34906..35431 Replicon plasmid pE1246_1
Accession NZ_CP025574
Organism Escherichia coli strain E-1246

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag CY655_RS27085 Protein ID WP_001159871.1
Coordinates 35126..35431 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag CY655_RS27080 Protein ID WP_000813634.1
Coordinates 34906..35124 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CY655_RS27060 31349..33487 + 2139 WP_032269946.1 ATPase AAA -
CY655_RS27065 33657..34058 - 402 WP_001441071.1 type II toxin-antitoxin system VapC family toxin -
CY655_RS27070 34082..34312 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CY655_RS27080 34906..35124 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CY655_RS27085 35126..35431 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
CY655_RS27090 35432..36241 + 810 WP_000016979.1 site-specific integrase -
CY655_RS27095 36379..36654 - 276 WP_000239529.1 hypothetical protein -
CY655_RS27100 36648..37292 - 645 WP_000633911.1 AAA family ATPase -
CY655_RS27105 37521..38492 + 972 WP_001103689.1 hypothetical protein -
CY655_RS27110 38497..38889 + 393 WP_000340832.1 plasmid partitioning/stability family protein -
CY655_RS27115 38921..40049 - 1129 Protein_48 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - agg3D / agg3C / agg3B / aap/aspU / pic 1..129195 129195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T90594 WP_001159871.1 NZ_CP025574:35126-35431 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T90594 NZ_CP025574:35126-35431 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT90594 WP_000813634.1 NZ_CP025574:34906-35124 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT90594 NZ_CP025574:34906-35124 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References