Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 33657..34312 Replicon plasmid pE1246_1
Accession NZ_CP025574
Organism Escherichia coli strain E-1246

Toxin (Protein)


Gene name vagD Uniprot ID A0A2B7QJJ1
Locus tag CY655_RS27065 Protein ID WP_001441071.1
Coordinates 33657..34058 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A0E0YAG4
Locus tag CY655_RS27070 Protein ID WP_001261288.1
Coordinates 34082..34312 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CY655_RS27045 28765..29190 + 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
CY655_RS27050 29187..29537 + 351 Protein_36 IS66 family insertion sequence element accessory protein TnpB -
CY655_RS27055 29568..31125 + 1558 Protein_37 IS66-like element ISEc23 family transposase -
CY655_RS27060 31349..33487 + 2139 WP_032269946.1 ATPase AAA -
CY655_RS27065 33657..34058 - 402 WP_001441071.1 type II toxin-antitoxin system VapC family toxin Toxin
CY655_RS27070 34082..34312 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CY655_RS27080 34906..35124 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
CY655_RS27085 35126..35431 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
CY655_RS27090 35432..36241 + 810 WP_000016979.1 site-specific integrase -
CY655_RS27095 36379..36654 - 276 WP_000239529.1 hypothetical protein -
CY655_RS27100 36648..37292 - 645 WP_000633911.1 AAA family ATPase -
CY655_RS27105 37521..38492 + 972 WP_001103689.1 hypothetical protein -
CY655_RS27110 38497..38889 + 393 WP_000340832.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - agg3D / agg3C / agg3B / aap/aspU / pic 1..129195 129195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14517.89 Da        Isoelectric Point: 7.2456

>T90593 WP_001441071.1 NZ_CP025574:c34058-33657 [Escherichia coli]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFGATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 402 bp

>T90593 NZ_CP025574:c34058-33657 [Escherichia coli]
ATGCTCGACACGAATATCTGCTCTTTCATTATGCGTGAACAGCCCGCCGCAGTGATCAAACGTCTGGAGCAGGCGGTGCT
GCGTAACCACCGCATTGTCGTGTCGGCCATCACCTATGCGGAGATGCGTTTCGGCGCCACCGGCCCGAAAGCCTCACCGC
GTCATATTGCGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATTCTGCCCTGGGACCGCGCTGCCGTGGACGCCACC
ACGGAGATTAAAATGGCACTGCGGCTGGCCGGGACGCCAATCGGCCCGAACGACACGGCGATTGCCGGGCACGCCATCGC
TGCCGGGGCGATACTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGGAAGACTGGGTGAAGT
AA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8684.76 Da        Isoelectric Point: 4.3654

>AT90593 WP_001261288.1 NZ_CP025574:c34312-34082 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL

Download         Length: 231 bp

>AT90593 NZ_CP025574:c34312-34082 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTTGAGATCGTCCGCGAAGGAGACAGCATTATATTGCGCCCGGTCCGGCCGACCTGGAGCTCGTTTGCGGCACTCG
AAAAGGCCGACGCAGACTTTATGGCGGAGCGTGAGGACATTGTCAGCGACGAAGGAAGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2B7QJJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0YAG4

References