90592

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 21986..22545 Replicon plasmid pE1246_1
Accession NZ_CP025574
Organism Escherichia coli strain E-1246

Toxin (Protein)


Gene name relE Uniprot ID A0A7B2ZE18
Locus tag CY655_RS26985 Protein ID WP_032250674.1
Coordinates 22270..22545 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag CY655_RS26980 Protein ID WP_001387467.1
Coordinates 21986..22270 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CY655_RS26955 17371..17937 - 567 Protein_20 zinc-binding dehydrogenase -
CY655_RS26960 18290..19180 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
CY655_RS26965 19205..19585 + 381 WP_001403366.1 Rid family detoxifying hydrolase -
CY655_RS26970 19618..20583 + 966 WP_023063778.1 2-keto-3-deoxygluconate transporter -
CY655_RS26975 20629..21381 - 753 WP_001403368.1 IclR family transcriptional regulator -
CY655_RS26980 21986..22270 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
CY655_RS26985 22270..22545 + 276 WP_032250674.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CY655_RS26990 22651..22944 + 294 WP_032284273.1 hypothetical protein -
CY655_RS26995 22979..23107 - 129 Protein_28 IS481 family transposase -
CY655_RS27000 23140..24309 - 1170 WP_032284272.1 hypothetical protein -
CY655_RS27010 24802..24972 + 171 WP_096790846.1 type II toxin-antitoxin system ParD family antitoxin -
CY655_RS27020 25225..25386 + 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
CY655_RS27025 25453..25635 + 183 WP_050488504.1 hypothetical protein -
CY655_RS27030 25756..26496 + 741 WP_001066941.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - agg3D / agg3C / agg3B / aap/aspU / pic 1..129195 129195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10750.48 Da        Isoelectric Point: 9.9324

>T90592 WP_032250674.1 NZ_CP025574:22270-22545 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGELLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T90592 NZ_CP025574:22270-22545 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACTGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT90592 WP_001387467.1 NZ_CP025574:21986-22270 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT90592 NZ_CP025574:21986-22270 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7B2ZE18

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References