Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 5170072..5170667 Replicon chromosome
Accession NZ_CP025573
Organism Escherichia coli strain E-1246

Toxin (Protein)


Gene name chpB Uniprot ID A0A0V9NWK6
Locus tag CY655_RS25485 Protein ID WP_019842229.1
Coordinates 5170317..5170667 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID L4JJX7
Locus tag CY655_RS25480 Protein ID WP_001223213.1
Coordinates 5170072..5170323 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CY655_RS25470 5165737..5169516 + 3780 WP_001550503.1 autotransporter assembly complex protein TamB -
CY655_RS25475 5169519..5169860 + 342 WP_001550504.1 gamma-glutamylcyclotransferase -
CY655_RS25480 5170072..5170323 + 252 WP_001223213.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
CY655_RS25485 5170317..5170667 + 351 WP_019842229.1 endoribonuclease toxin ChpB Toxin
CY655_RS25490 5170871..5171401 - 531 WP_000055075.1 inorganic diphosphatase -
CY655_RS25495 5171711..5172667 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
CY655_RS25500 5172799..5174301 + 1503 WP_001550506.1 sugar ABC transporter ATP-binding protein -
CY655_RS25505 5174315..5175337 + 1023 WP_001550507.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-115)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12483.44 Da        Isoelectric Point: 5.6097

>T90588 WP_019842229.1 NZ_CP025573:5170317-5170667 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVWMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Download         Length: 351 bp

>T90588 NZ_CP025573:5170317-5170667 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9331.64 Da        Isoelectric Point: 4.6509

>AT90588 WP_001223213.1 NZ_CP025573:5170072-5170323 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT90588 NZ_CP025573:5170072-5170323 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NWK6

Antitoxin

Source ID Structure
AlphaFold DB L4JJX7

References