Detailed information of TA system TA090583

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3723839..3724671
• Replicon: chromosome
• Accession: NZ_CP025573
• Organism: Escherichia coli strain E-1246

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: CY655_RS18610
• Protein ID: WP_000854765.1
• Coordinates: 3724297..3724671 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: CY655_RS18605
• Protein ID: WP_001295723.1
• Coordinates: 3723839..3724207 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY655_RS18570	3719503..3720183	+	681	WP_001282921.1	WYL domain-containing protein	-
CY655_RS18575	3720331..3721008	+	678	WP_001097302.1	hypothetical protein	-
CY655_RS18580	3721014..3721247	+	234	WP_001278293.1	DUF905 family protein	-
CY655_RS18585	3721337..3722155	+	819	WP_001175165.1	DUF945 domain-containing protein	-
CY655_RS18590	3722421..3722900	+	480	WP_057109541.1	antirestriction protein	-
CY655_RS18595	3722916..3723392	+	477	WP_001186774.1	RadC family protein	-
CY655_RS18600	3723455..3723676	+	222	WP_000692341.1	DUF987 domain-containing protein	-
CY655_RS18605	3723839..3724207	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CY655_RS18610	3724297..3724671	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
CY655_RS18615	3724671..3724874	+	204	WP_001398797.1	hypothetical protein	-
CY655_RS18620	3724899..3725093	+	195	WP_024181941.1	DUF957 domain-containing protein	-
CY655_RS18625	3725178..3726020	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
CY655_RS18630	3726363..3726533	+	171	Protein_3553	IS110 family transposase	-
CY655_RS18635	3727343..3728326	+	984	WP_001376028.1	KpsF/GutQ family sugar-phosphate isomerase	-
CY655_RS18640	3728398..3729546	+	1149	WP_000905929.1	capsule polysaccharide transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T90583 WP_000854765.1 NZ_CP025573:3724297-3724671 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T90583 NZ_CP025573:3724297-3724671 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT90583 WP_001295723.1 NZ_CP025573:3723839-3724207 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT90583 NZ_CP025573:3723839-3724207 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52