Detailed information of TA system TA090569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1785490..1786128
• Replicon: chromosome
• Accession: NZ_CP025573
• Organism: Escherichia coli strain E-1246

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: CY655_RS08875
• Protein ID: WP_000813795.1
• Coordinates: 1785490..1785666 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CY655_RS08880
• Protein ID: WP_076797675.1
• Coordinates: 1785712..1786128 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY655_RS08855	1781112..1782323	-	1212	WP_071591517.1	BenE family transporter YdcO	-
CY655_RS08860	1782376..1782912	+	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
CY655_RS08865	1782985..1784946	+	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CY655_RS08870	1785038..1785268	-	231	WP_023910283.1	YncJ family protein	-
CY655_RS08875	1785490..1785666	+	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CY655_RS08880	1785712..1786128	+	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CY655_RS08885	1786207..1787613	+	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
CY655_RS08890	1787858..1789003	+	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
CY655_RS08895	1789021..1790034	+	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
CY655_RS08900	1790035..1790976	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T90569 WP_000813795.1 NZ_CP025573:1785490-1785666 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T90569 NZ_CP025573:1785490-1785666 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT90569 WP_076797675.1 NZ_CP025573:1785712-1786128 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT90569 NZ_CP025573:1785712-1786128 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1