Detailed information of TA system TA090566

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1268083..1268927
• Replicon: chromosome
• Accession: NZ_CP025573
• Organism: Escherichia coli strain E-1246

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: CY655_RS05950
• Protein ID: WP_000854686.1
• Coordinates: 1268544..1268927 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: CY655_RS05945
• Protein ID: WP_001285602.1
• Coordinates: 1268083..1268463 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY655_RS05905	1264332..1264787	+	456	WP_001504120.1	hypothetical protein	-
CY655_RS05910	1264866..1265099	+	234	WP_001119719.1	DUF905 family protein	-
CY655_RS05920	1265199..1266017	+	819	WP_001234732.1	DUF945 domain-containing protein	-
CY655_RS05925	1266109..1266594	+	486	WP_000214307.1	antirestriction protein	-
CY655_RS05930	1266610..1267086	+	477	WP_001186726.1	RadC family protein	-
CY655_RS05935	1267149..1267370	+	222	WP_000692298.1	DUF987 domain-containing protein	-
CY655_RS05940	1267389..1268072	+	684	WP_000086768.1	hypothetical protein	-
CY655_RS05945	1268083..1268463	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CY655_RS05950	1268544..1268927	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
CY655_RS05955	1268924..1269412	+	489	WP_001054233.1	hypothetical protein	-
CY655_RS05960	1269429..1269626	+	198	WP_000839253.1	DUF957 domain-containing protein	-
CY655_RS05965	1269711..1270553	+	843	WP_001431817.1	DUF4942 domain-containing protein	-
CY655_RS05975	1271024..1271962	+	939	WP_001550942.1	glyoxylate/hydroxypyruvate reductase GhrA	-
CY655_RS05980	1272017..1272754	+	738	WP_000283664.1	zinc-binding phosphatase	-
CY655_RS05985	1272778..1273332	+	555	WP_001001909.1	molecular chaperone YcdY	-
CY655_RS05990	1273434..1273925	+	492	WP_032145787.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T90566 WP_000854686.1 NZ_CP025573:1268544-1268927 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T90566 NZ_CP025573:1268544-1268927 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT90566 WP_001285602.1 NZ_CP025573:1268083-1268463 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT90566 NZ_CP025573:1268083-1268463 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG