Detailed information of TA system TA090565

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1017742..1018537
• Replicon: chromosome
• Accession: NZ_CP025573
• Organism: Escherichia coli strain E-1246

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: CY655_RS04740
• Protein ID: WP_000854914.1
• Coordinates: 1018163..1018537 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7UP42
• Locus tag: CY655_RS04735
• Protein ID: WP_001280955.1
• Coordinates: 1017742..1018116 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY655_RS04700	1013097..1014002	+	906	WP_000203541.1	chemotaxis protein	-
CY655_RS04705	1013999..1015069	+	1071	WP_000102669.1	patatin-like phospholipase family protein	-
CY655_RS04715	1015409..1016227	+	819	WP_016235315.1	DUF945 domain-containing protein	-
CY655_RS04720	1016318..1016803	+	486	WP_000214398.1	antirestriction protein	-
CY655_RS04725	1016819..1017295	+	477	WP_001186738.1	RadC family protein	-
CY655_RS04730	1017358..1017579	+	222	WP_001220314.1	DUF987 domain-containing protein	-
CY655_RS04735	1017742..1018116	+	375	WP_001280955.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CY655_RS04740	1018163..1018537	+	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
CY655_RS04745	1018534..1019025	+	492	WP_000976857.1	hypothetical protein	-
CY655_RS04750	1019037..1019234	+	198	WP_000839282.1	DUF957 domain-containing protein	-
CY655_RS04755	1019319..1020161	+	843	WP_016231095.1	DUF4942 domain-containing protein	-
CY655_RS04765	1020652..1020870	-	219	WP_001040187.1	translation initiation factor IF-1	-
CY655_RS04770	1021155..1021859	-	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T90565 WP_000854914.1 NZ_CP025573:1018163-1018537 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T90565 NZ_CP025573:1018163-1018537 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13767.59 Da
• Isoelectric Point: 6.6248

>AT90565 WP_001280955.1 NZ_CP025573:1017742-1018116 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT90565 NZ_CP025573:1017742-1018116 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A7U9P0D0