Detailed information of TA system TA090552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 6190950..6191520
• Replicon: chromosome
• Accession: NZ_CP025551
• Organism: Streptomyces rimosus strain WT5260

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: L8ERH5
• Locus tag: CTZ40_RS26825
• Protein ID: WP_003982453.1
• Coordinates: 6191173..6191520 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: L8ERT3
• Locus tag: CTZ40_RS26820
• Protein ID: WP_003982452.1
• Coordinates: 6190950..6191186 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTZ40_RS26805	6186372..6187529	+	1158	WP_003982449.1	allantoicase	-
CTZ40_RS26810	6187601..6190177	-	2577	WP_003982450.1	aminopeptidase N	-
CTZ40_RS26815	6190311..6190910	+	600	WP_003982451.1	Uma2 family endonuclease	-
CTZ40_RS26820	6190950..6191186	+	237	WP_003982452.1	ribbon-helix-helix domain-containing protein	Antitoxin
CTZ40_RS26825	6191173..6191520	+	348	WP_003982453.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CTZ40_RS26830	6191601..6192122	+	522	WP_003982454.1	DUF1203 domain-containing protein	-
CTZ40_RS26835	6192193..6193224	-	1032	WP_003982455.1	aspartate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12493.43 Da
• Isoelectric Point: 10.8691

>T90552 WP_003982453.1 NZ_CP025551:6191173-6191520 [Streptomyces rimosus]
MRRGDLYLVDLEPVRGSEANKYRPAVVVSNDAANRSVQRAGRGVVTVVPVTANVARVYPFQVLLTADDCGLPRDSKAQCE
QVRAVSVERLGRRVGSVPARVMVGLDAALRRHLAL

Nucleotide

• Length: 348 bp

>T90552 NZ_CP025551:6191173-6191520 [Streptomyces rimosus]
ATGCGGCGCGGTGACCTCTATCTGGTTGATCTGGAGCCGGTCCGGGGGAGCGAGGCCAACAAGTACCGTCCTGCGGTGGT
CGTGTCCAACGACGCGGCCAATCGGTCCGTGCAGCGCGCCGGAAGGGGTGTGGTGACGGTCGTGCCTGTGACGGCCAACG
TCGCCCGGGTCTACCCGTTCCAGGTGCTGCTGACCGCCGACGACTGCGGGCTGCCGCGCGACTCGAAGGCCCAGTGCGAG
CAGGTGCGCGCGGTGTCCGTCGAGCGGCTGGGGCGGCGGGTCGGCAGCGTCCCGGCGCGCGTGATGGTTGGGCTGGACGC
GGCCCTGCGCAGGCATCTGGCGCTCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8374.07 Da
• Isoelectric Point: 3.7856

>AT90552 WP_003982452.1 NZ_CP025551:6190950-6191186 [Streptomyces rimosus]
MKISVSLPEEDIAFLDAYGKKTDADSRSAVIHAAVELLRASELEADYASAWEEWDESDDALLWDGVSGDGIGSADAAR

Nucleotide

• Length: 237 bp

>AT90552 NZ_CP025551:6190950-6191186 [Streptomyces rimosus]
ATGAAGATCAGCGTCAGCCTGCCGGAAGAGGACATCGCCTTCCTCGACGCGTACGGCAAGAAGACGGACGCCGATTCGCG
GTCGGCGGTGATCCACGCGGCGGTCGAGCTGCTGCGTGCCTCCGAGCTGGAGGCGGATTACGCCTCGGCGTGGGAGGAGT
GGGACGAGAGCGACGACGCGCTGCTGTGGGACGGGGTCTCCGGGGACGGGATCGGAAGCGCCGATGCGGCGCGGTGA

Structures

Toxin

• AlphaFold DB: L8ERH5

Antitoxin

• AlphaFold DB: L8ERT3