Detailed information of TA system TA090550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 41791..42389
• Replicon: plasmid unnamed1
• Accession: NZ_CP025547
• Organism: Mycobacterium paragordonae strain 49061

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C0J29_RS30410
• Protein ID: WP_084023347.1
• Coordinates: 42045..42389 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C0J29_RS30405
• Protein ID: WP_120795059.1
• Coordinates: 41791..42051 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0J29_RS30380	37039..37542	+	504	WP_162951649.1	helix-turn-helix domain-containing protein	-
C0J29_RS30385	37578..38276	+	699	WP_162951650.1	hypothetical protein	-
C0J29_RS30390	38340..38600	+	261	WP_139809397.1	hypothetical protein	-
C0J29_RS33070	38629..38937	+	309	WP_139809396.1	hypothetical protein	-
C0J29_RS33075	38999..39220	+	222	WP_139809395.1	hypothetical protein	-
C0J29_RS30395	39404..39811	-	408	WP_139809394.1	hypothetical protein	-
C0J29_RS30400	40643..41536	+	894	WP_084023351.1	hypothetical protein	-
C0J29_RS30405	41791..42051	+	261	WP_120795059.1	ribbon-helix-helix domain-containing protein	Antitoxin
C0J29_RS30410	42045..42389	+	345	WP_084023347.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C0J29_RS30415	43035..43646	+	612	WP_120795128.1	transglycosylase SLT domain-containing protein	-
C0J29_RS30420	43676..44167	+	492	WP_162951651.1	hypothetical protein	-
C0J29_RS30425	44785..45336	+	552	WP_120795129.1	hypothetical protein	-
C0J29_RS30430	45333..46865	+	1533	WP_120795061.1	type VII secretion protein EccB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	esxN / eccA5	1..305730	305730

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12048.89 Da
• Isoelectric Point: 9.8887

>T90550 WP_084023347.1 NZ_CP025547:42045-42389 [Mycobacterium paragordonae]
VVIGRAEIYWADLGAPSGSRPGKRRPVAVIQSDPYNASRLATVLAAVITSNTGLAAMPGNVFLPASTTGLSRDSVVNVTA
LVTLNKTDLIDRIGEIPASLMHEVDRGLRRVLDL

Nucleotide

• Length: 345 bp

>T90550 NZ_CP025547:42045-42389 [Mycobacterium paragordonae]
GTGGTGATCGGCCGCGCAGAGATCTACTGGGCCGACCTCGGCGCCCCCTCCGGCAGCCGACCCGGAAAGCGTCGTCCCGT
AGCAGTGATCCAATCCGATCCCTACAACGCCAGTCGCCTCGCCACGGTGCTCGCCGCCGTGATCACCTCCAACACGGGGC
TAGCAGCCATGCCAGGCAACGTGTTCCTACCTGCGTCCACAACGGGACTGTCACGTGATTCGGTCGTCAACGTCACGGCA
CTTGTGACGCTGAACAAGACCGACCTCATCGATCGCATCGGGGAGATACCGGCGAGCTTGATGCACGAAGTCGACCGCGG
ACTTCGTCGCGTACTCGACCTTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9481.34 Da
• Isoelectric Point: 4.2883

>AT90550 WP_120795059.1 NZ_CP025547:41791-42051 [Mycobacterium paragordonae]
MKTAISLPDETFDRASRRARDLGMSRSEFFTRAAQRYLDELDSQSLTGQIDDALEHLGERLGDSDGGQAAAVAVGHRVLD
AVDDEW

Nucleotide

• Length: 261 bp

>AT90550 NZ_CP025547:41791-42051 [Mycobacterium paragordonae]
ATGAAGACCGCCATCTCCCTACCCGATGAGACGTTCGATCGCGCTTCGCGCCGGGCCCGTGACCTCGGCATGAGCCGGTC
GGAGTTCTTCACCCGGGCCGCGCAGCGCTACCTCGACGAGTTGGACAGCCAATCGCTGACCGGGCAGATCGACGACGCTC
TTGAGCATCTTGGTGAGCGGCTTGGTGACAGCGATGGGGGGCAAGCCGCAGCGGTGGCCGTCGGGCACCGGGTGCTTGAC
GCCGTGGACGACGAGTGGTGA