Detailed information of TA system TA090549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 5694354..5695011
• Replicon: chromosome
• Accession: NZ_CP025546
• Organism: Mycobacterium paragordonae strain 49061

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: A0A386UAJ3
• Locus tag: C0J29_RS25380
• Protein ID: WP_065049810.1
• Coordinates: 5694354..5694827 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: C0J29_RS25385
• Protein ID: WP_055581736.1
• Coordinates: 5694832..5695011 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0J29_RS25355	5689727..5690200	+	474	WP_065049803.1	VOC family protein	-
C0J29_RS25360	5690271..5691581	-	1311	WP_120793931.1	adenylate/guanylate cyclase domain-containing protein	-
C0J29_RS25365	5691591..5692025	-	435	WP_065049807.1	hypothetical protein	-
C0J29_RS25370	5692054..5692827	-	774	WP_065165638.1	VOC family protein	-
C0J29_RS25375	5693063..5694271	+	1209	WP_172834827.1	IS110 family transposase	-
C0J29_RS25380	5694354..5694827	-	474	WP_065049810.1	SRPBCC family protein	Toxin
C0J29_RS25385	5694832..5695011	-	180	WP_055581736.1	antitoxin	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5693069..5694271	1202

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17315.08 Da
• Isoelectric Point: 10.4094

>T90549 WP_065049810.1 NZ_CP025546:c5694827-5694354 [Mycobacterium paragordonae]
MAKLSGSIDVPLPPEQAWTHASDLNRYREWLTIHKVWRSKLPDVLEKGTVIESYVEVKGMPNRIRWTIVRYKPPEGMTLN
GDGVGGVKVKLMAKVAPKEQGSVVSFDVHLGGPALFGPIGMVVAAALRSDIRDSLQNFVRVFARPDPGTNGDSVLRR

Nucleotide

• Length: 474 bp

>T90549 NZ_CP025546:c5694827-5694354 [Mycobacterium paragordonae]
ATGGCGAAACTCTCCGGATCCATCGATGTGCCGCTGCCGCCGGAACAGGCCTGGACACACGCCTCCGACCTGAACCGGTA
CCGGGAATGGCTGACCATCCACAAGGTCTGGCGCAGCAAGTTGCCCGACGTGCTGGAAAAGGGCACCGTCATCGAGTCCT
ATGTCGAAGTCAAGGGCATGCCCAACCGCATCAGGTGGACGATCGTGCGCTACAAGCCCCCGGAGGGCATGACCCTCAAC
GGCGACGGTGTGGGCGGGGTCAAGGTGAAACTGATGGCCAAGGTCGCTCCGAAAGAGCAGGGTTCGGTCGTCAGCTTCGA
CGTGCATCTCGGCGGGCCGGCGCTGTTCGGGCCGATCGGCATGGTCGTCGCCGCCGCGCTGCGCAGCGATATCCGCGACT
CGCTGCAGAACTTCGTCAGGGTGTTCGCCCGCCCGGACCCGGGGACGAACGGCGACAGCGTCCTGCGTCGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6483.23 Da
• Isoelectric Point: 4.9849

>AT90549 WP_055581736.1 NZ_CP025546:c5695011-5694832 [Mycobacterium paragordonae]
MAFLDKAKDLLAQNADKVETAITKAGEFVDDKTQGKYSDTIHKVQEEARKIVDTGGQQS

Nucleotide

• Length: 180 bp

>AT90549 NZ_CP025546:c5695011-5694832 [Mycobacterium paragordonae]
ATGGCATTCCTGGACAAGGCCAAAGACCTGCTCGCGCAGAACGCCGACAAGGTCGAGACGGCGATCACCAAGGCCGGCGA
GTTCGTCGACGACAAGACACAGGGCAAGTACTCCGACACCATCCACAAGGTGCAGGAGGAAGCCAGGAAGATCGTCGACA
CCGGCGGCCAGCAGAGCTGA

Structures

Toxin

• AlphaFold DB: A0A386UAJ3