Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1322264..1322883 Replicon chromosome
Accession NZ_CP025541
Organism Klebsiella pneumoniae strain 2N3

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CY657_RS06260 Protein ID WP_002892050.1
Coordinates 1322264..1322482 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CY657_RS06265 Protein ID WP_002892066.1
Coordinates 1322509..1322883 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CY657_RS06230 1318311..1318574 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
CY657_RS06235 1318574..1318714 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CY657_RS06240 1318711..1319409 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
CY657_RS06245 1319510..1320961 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CY657_RS06250 1320936..1321406 - 471 WP_002892026.1 YlaC family protein -
CY657_RS26230 1321427..1321567 + 141 WP_004147370.1 hypothetical protein -
CY657_RS06255 1321539..1322105 - 567 WP_002892030.1 maltose O-acetyltransferase -
CY657_RS06260 1322264..1322482 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CY657_RS06265 1322509..1322883 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CY657_RS06270 1323369..1326515 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CY657_RS06275 1326538..1327731 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T90531 WP_002892050.1 NZ_CP025541:c1322482-1322264 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T90531 NZ_CP025541:c1322482-1322264 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT90531 WP_002892066.1 NZ_CP025541:c1322883-1322509 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT90531 NZ_CP025541:c1322883-1322509 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References